The role of β(2) integrin in dendritic cell migration during infection.
The role of β(2) integrin in dendritic cell migration during infection.
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DOI：
10.1186/s12865-020-00394-5
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发表时间：
2021-01-06
期刊：
影响因子：
3
通讯作者：
Cruickshank S
中科院分区：
文献类型：
--
作者：
Altorki T;Muller W;Brass A;Cruickshank S
树突状细胞（DC）在塑造T细胞反应中起关键作用。要做到这一点，DC必须能够迁移到感染部位和淋巴结，以引发T细胞并启动适当的免疫应答。整联蛋白如β2整联蛋白在白细胞粘附、迁移和细胞活化中起关键作用。然而，β2整联蛋白在肠道感染诱导的炎症背景下DC迁移和功能中的作用还不清楚。本研究观察了β2整合素在小鼠鞭虫感染过程中DC迁移和功能中的作用。将缺乏功能性β2整联蛋白的Itgb 2 tm 1Bay小鼠和WT同窝对照感染T.小鼠和对感染的反应和DC反应的动力学进行了评估。在感染中，功能性β2整合素的缺乏显著减少了DC向感染部位的迁移，但对淋巴结没有影响。功能性β2整合素的缺乏对T细胞活化没有负面影响。小鼠感染。这些数据表明，β2整联蛋白在DC募集到感染部位中是重要的，可能影响先天免疫的启动，但在T细胞的背景下，对于DC迁移到淋巴结和T细胞引发是不可或缺的。小鼠感染。在线版本包含补充材料，可通过10.1186/s12865-020-00394-5获得。
Dendritic cells (DCs) play a key role in shaping T cell responses. To do this, DCs must be able to migrate to the site of the infection and the lymph nodes to prime T cells and initiate the appropriate immune response. Integrins such as β2 integrin play a key role in leukocyte adhesion, migration, and cell activation. However, the role of β2 integrin in DC migration and function in the context of infection-induced inflammation in the gut is not well understood. This study looked at the role of β2 integrin in DC migration and function during infection with the nematode worm Trichuris muris. Itgb2tm1Bay mice lacking functional β2 integrin and WT littermate controls were infected with T. muris and the response to infection and kinetics of the DC response was assessed. In infection, the lack of functional β2 integrin significantly reduced DC migration to the site of infection but not the lymph nodes. The lack of functional β2 integrin did not negatively impact T cell activation in response to T. muris infection. This data suggests that β2 integrins are important in DC recruitment to the infection site potentially impacting the initiation of innate immunity but is dispensible for DC migration to lymph nodes and T cell priming in the context of T. muris infection. The online version contains supplementary material available at 10.1186/s12865-020-00394-5.