Antiproliferative effect of Tualang honey on oral squamous cell carcinoma and osteosarcoma cell lines
Antiproliferative effect of Tualang honey on oral squamous cell carcinoma and osteosarcoma cell lines
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DOI：
10.1186/1472-6882-10-49
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发表时间：
2010-09-14
影响因子：
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通讯作者：
Saini, Rajan
中科院分区：
文献类型：
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作者：
Ghashm, Abdulmlik A.;Othman, Nor H.;Saini, Rajan
背景资料：口腔鳞状细胞癌（OSCC）和人骨肉瘤（HOS）的治疗包括手术和/或放疗，这通常会导致生活质量下降。方法：将土阿朗蜂蜜（1%~ 20%）分别作用于口腔鳞癌（OSCC）和口腔鳞状细胞癌（HOS）细胞株3、6、12、24、48和72 h，观察土阿朗蜂蜜对OSCC和HOS细胞株增殖的抑制作用。光镜和荧光显微镜下观察形态学特征。使用MTT测定法评估细胞活力，并通过ELISA读数器在570 nm处测量每个实验中吸光度值的光密度。结果：OSCC和HOS细胞形态学表现为凋亡细胞变圆、细胞数量减少、细胞膜出现泡状改变，细胞核出现皱缩、染色质浓缩、核碎裂等凋亡性改变。细胞活力测定显示蜂蜜对两种细胞系的抑制作用具有时间和剂量依赖性。对口腔鳞状细胞癌和口腔鳞状细胞癌细胞系的半数抑制浓度（IC_（50））分别为4%和3.5%。对于两种细胞系，在15%时获得>= 80%的最大细胞生长抑制。结论：土郎蜂蜜通过诱导早期凋亡抑制OSCC和HOS细胞的增殖，其作用机制可能与其诱导早期凋亡有关。
Background: The treatment of oral squamous cell carcinomas (OSCC) and human osteosarcoma (HOS) includes surgery and/or radiotherapy which often lead to reduced quality of life. This study was aimed to study the antiproliferative activity of local honey (Tualang) on OSCC and HOS cell lines.Methods: Several concentrations of Tualang honey (1% - 20%) were applied on OSCC and HOS cell lines for 3, 6, 12, 24, 48 and 72 hours. Morphological characteristics were observed under light and fluorescent microscope. Cell viability was assessed using MTT assay and the optical density for absorbance values in each experiment was measured at 570 nm by an ELISA reader. Detection of cellular apoptosis was done using the Annexin V-FITC Apoptosis Detection Kit.Results: Morphological appearance showed apoptotic cellular changes like becoming rounded, reduction in cell number, blebbed membrane and apoptotic nuclear changes like nuclear shrinkage, chromatin condensation and fragmented nucleus on OSCC and HOS cell lines. Cell viability assay showed a time and dose-dependent inhibitory effect of honey on both cell lines. The 50% inhibitory concentration (IC50) for OSCC and HOS cell lines was found to be 4% and 3.5% respectively. The maximum inhibition of cell growth of >= 80% was obtained at 15% for both cell lines. Early apoptosis was evident by flow cytometry where percentage of early apoptotic cells increased in dose and time dependent manner.Conclusion: Tualang honey showed antiproliferative effect on OSCC and HOS cell lines by inducing early apoptosis.