Epstein-Barr Virus and Human Disease
Epstein-Barr Virus and Human Disease
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EB 病毒与人类疾病
DOI：
10.1007/978-1-4612-4590-2
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发表时间：
1987
影响因子：
2.7
通讯作者：
R. Glaser
中科院分区：
文献类型：
--
作者：
Paul H. Levine;D. Ablashi;M. Nonoyama;G. R. Pearson;R. Glaser
我们将P3 HR-1缺陷DNA编码的产物之一鉴定为45/60 kd多肽家族，其映射到BHLF 1/BSLF 2开放阅读框。我们将此产品命名为HS-EA。在瞬时表达试验中，发现野生型和缺陷型DNA编码的HS-EA都能激活多种启动子-CAT构建体的表达。因此，HS-EA具有广义反式激活剂的性质。用P3 HR-1病毒超感染潜伏EBV感染的Raji细胞系导致Raji细胞中裂解周期表达的诱导。P3 HR-1的无缺陷克隆分离物不能诱导Raji中的早期抗原（EA）表达，这暗示缺陷DNA群体作为诱导剂（1）。Raji细胞中缺陷DNA诱导的裂解基因表达为病毒从潜伏期重新激活提供了模型系统。考虑到这一点，我们克隆了主要的P3 HR-1缺陷DNA种类，并试图在缺陷DNA克隆中鉴定其产物可能在裂解诱导过程中起作用的基因。用缺陷DNA克隆转染幼仓鼠肾细胞，并用表征的人血清染色。该程序确定了一个核
We identified one of the products encoded by P3HR-l defective DNA as a 45/60 kd family of polypeptides which mapped to the BHLF1/BSLF2 open reading frames. We termed this product HS-EA. In transient expression assays, both wild-type and defective DNA encoded HS-EA were found to activate expression from a variety of promoter-CAT constructs. Thus HS-EA has the properties of a generalized trans-activator. Superinfection of the latently EBV-infected Raji cell line with P3HR-l virus results in the induction of lytic cycle expression in the Raji cells. Defective-free clonal isolates of P3HR-l are unable to induce early antigen (EA) expression in Raji which implicates the defective DNA population as agents for the induction (1). The defective DNA induced lytic gene expression in Raji cells provides a model system for reactivation of virus from latency. With this in mind, we cloned the major P3HR-l defective DNA species and sought to identify within the defective DNA clones genes whose products might play a role in the lytic induction process. Baby hamster kidney cells were transfected with defective DNA clones and stained with characterized human sera. This procedure identified a nuclear