EXPRESSION OF LIGHT-HARVESTING CHLOROPHYLL A/B-PROTEIN GENES IS PHYTOCHROME-REGULATED IN ETIOLATED ARABIDOPSIS-THALIANA SEEDLINGS
EXPRESSION OF LIGHT-HARVESTING CHLOROPHYLL A/B-PROTEIN GENES IS PHYTOCHROME-REGULATED IN ETIOLATED ARABIDOPSIS-THALIANA SEEDLINGS
复制标题
DOI：
10.1104/pp.88.4.1323
复制
发表时间：
1988-12-01
期刊：
影响因子：
7.4
通讯作者：
TOBIN, EM
中科院分区：
文献类型：
--
作者：
KARLINNEUMANN, GA;SUN, L;TOBIN, EM
光敏色素作用导致拟南芥黄化幼苗中捕光叶绿素a/b蛋白（LHCP）mRNA水平的大而快速的增加：在1分钟红光照射后1小时内可检测到RNA的增加，在约10分钟时达到最大30倍，高于黑暗水平。2小时，并且在短暂的红色照射后约8小时衰减回暗水平。S1核酸酶分析区分了从先前在拟南芥中表征的LHCP基因家族的三个成员转录的两种mRNA（LS Leutwiler，EM Meyerowitz，EM Tobin，1986 Nucleic Acids Res 14：4051-4064）。其中一个来自AB 140基因，而另一个代表AB 165和/或AB 180基因的产物（AB 165/AB 180 mRNA）。在成熟的白色光照下生长的植物中，这两种mRNA的含量几乎相等。相比之下，在黄化幼苗，1分钟的红光引起的AB 140 mRNA的水平比AB 165/AB 180 mRNA的水平增加6倍，虽然这两个水平的光敏色素的作用。两种转录本对1分钟红光的响应动力学相似。其他证据表明，这种差异表达是发育调节。由于AB 140基因为进一步分析拟南芥中光敏色素调控的基因表达提供了一个有吸引力的靶点，我们通过定位其5“和3”转录物末端来进一步表征该基因。
Phytochrome action results in a large and rapid increase in the light-harvesting chlorophyll a/b-protein (LHCP) mRNA level in etiolated seedlings of Arabidopsis thaliana: the RNA increase is detectable within 1 hour after 1 minute red illumination, reaches a maximum 30-fold higher than the dark level at ca. 2 hours, and decays back to dark levels by about 8 hours after the brief red illumination. S1 nuclease analysis distinguishes two kinds of mRNAs transcribed from the three members of the LHCP gene family previously characterized for Arabidopsis (LS Leutwiler, EM Meyerowitz, EM Tobin, 1986 Nucleic Acids Res 14: 4051-4064). One of these arises from the AB140 gene, while the other represents the product(s) of the AB165 and/or AB180 gene(s) (AB165/AB180 mRNA). In mature, white light-grown plants, the two kinds of mRNAs are present in nearly equal amounts. In contrast, in etiolated seedlings, 1 minute red light causes a sixfold greater increase in the level of AB140 mRNA than in the level of AB165/AB180 mRNA, although both levels are regulated by phytochrome action. The kinetics of the responses to 1 minute red light are similar for both kinds of transcripts. Additional evidence suggests that this differential expression is developmentally regulated. Because the AB140 gene offers an attractive target for further analysis of phytochrome-regulated gene expression in Arabidopsis, we have further characterized this gene by mapping its 5'' and 3'' transcript termini.