SEQUENTIAL-ANALYSIS OF GENE-EXPRESSION AFTER AN OSTEOGENIC STIMULUS - C-FOS EXPRESSION IS INDUCED IN OSTEOCYTES
SEQUENTIAL-ANALYSIS OF GENE-EXPRESSION AFTER AN OSTEOGENIC STIMULUS - C-FOS EXPRESSION IS INDUCED IN OSTEOCYTES
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DOI：
10.1006/bbrc.1995.2773
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发表时间：
1995-12-05
影响因子：
3.1
通讯作者：
CHAMBERS, TJ
中科院分区：
文献类型：
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作者：
INAOKA, T;LEAN, JM;CHAMBERS, TJ
我们最近开发了一种实验模型，其中机械刺激诱导大鼠尾椎成骨。我们使用该模型来评估机械负荷诱导的基因表达。在北方印迹分析中，上样后提取的mRNA的大量制备物未显示基质蛋白或生长因子的mRNA表达增加>2倍。c-jun是检测不到的。然而，c-fos在加载的60分钟内表现出4倍的表达增加，然后在4小时后恢复到对照水平。这种增加与原位杂交中的强烈信号有关，在任何未加载的椎骨中看不到，对于皮质骨细胞的c-fos：因此骨细胞对机械加载的c-fos表达的反应如此强烈，以至于即使在散装RNA制剂中也可见。这些结果代表了骨细胞和c-fos在骨对机械刺激的成骨反应中的作用的有说服力的证据。(C)出版社：Academic Press
We have recently developed an experimental model whereby mechanical stimulation induces osteogenesis in the caudal vertebrae of rats. We used this model to assess expression of genes induced by mechanical loading. Bulk preparations of mRNA extracted after loading did not-show >2-fold increases in expression of mRNA for matrix proteins or growth factors in Northern blotting analysis. c-jun was undetectable. However, c-fos showed a 4-fold increase in expression within 60 mins of loading, before returning to control levels by 4 hrs. This increase was associated with intense signals in in situ hybridization, not seen in any nonloaded vertebrae, for c-fos over cortical osteocytes: thus osteocytes respond to mechanical loading with c-fos expression so strongly as to be visible even in the bulk RNA preparations. The results represent persuasive evidence for a role for osteocytes, and for c-fos, in the osteogenic response of bone to mechanical stimulation. (C) 1995 Academic Press, Inc.