Functional distinctness in the exoproteomes of marine Synechococcus.
Functional distinctness in the exoproteomes of marine Synechococcus.
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DOI：
10.1111/1462-2920.12822
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发表时间：
2015-10
影响因子：
5.1
通讯作者：
Scanlan DJ
中科院分区：
文献类型：
--
作者：
Christie-Oleza JA;Armengaud J;Guerin P;Scanlan DJ
一个生物体的分泌蛋白组分可能反映其生存策略，并最终反映其被外界感知的方式。对原绿球藻和聚球藻谱系的分泌全蛋白质组的生物信息学预测表明：（i）编码蛋白质组的这一组分中谱系特异性蛋白质的发生率比胞质组分高得多（同源发生率分别为57%和73%）；（ii）与胞质组分的蛋白质（35%）相比，迄今为止分泌蛋白在很大程度上尚未被鉴定（54%）。这表明这些生物体的基因组和功能多样性主要在于它们分泌到细胞膜或通过细胞膜的新功能的多样化集合，这在群落多样化中起着关键作用，例如在生态位分化或逃避捕食方面。对海洋聚球藻的实验性胞外蛋白质组分析显示了无机营养物质的转运系统、一系列有趣的参与互利或敌对相互作用的菌株特异性胞外蛋白（即溶血素、菌毛蛋白、黏附素）以及具有潜在混合营养目标的胞外酶（即胞外蛋白酶和几丁质酶）。我们还展示了这些生物体如何重塑它们的胞外蛋白质组，即当在异养生物存在下生长时增加相互作用蛋白质的种类，或者当在黑暗中生长时减少对猎物的暴露。最后，我们的数据表明异养细菌可以以聚球藻的胞外蛋白质组为食。
The exported protein fraction of an organism may reflect its life strategy and, ultimately, the way it is perceived by the outside world. Bioinformatic prediction of the exported pan‐proteome of P rochlorococcus and S ynechococcus lineages demonstrated that (i) this fraction of the encoded proteome had a much higher incidence of lineage‐specific proteins than the cytosolic fraction (57% and 73% homologue incidence respectively) and (ii) exported proteins are largely uncharacterized to date (54%) compared with proteins from the cytosolic fraction (35%). This suggests that the genomic and functional diversity of these organisms lies largely in the diverse pool of novel functions these organisms export to/through their membranes playing a key role in community diversification, e.g. for niche partitioning or evading predation. Experimental exoproteome analysis of marine S ynechococcus showed transport systems for inorganic nutrients, an interesting array of strain‐specific exoproteins involved in mutualistic or hostile interactions (i.e. hemolysins, pilins, adhesins), and exoenzymes with a potential mixotrophic goal (i.e. exoproteases and chitinases). We also show how these organisms can remodel their exoproteome, i.e. by increasing the repertoire of interaction proteins when grown in the presence of a heterotroph or decrease exposure to prey when grown in the dark. Finally, our data indicate that heterotrophic bacteria can feed on the exoproteome of S ynechococcus.