56Fe Irradiation Alters Spine Density and Dendritic Complexity in the Mouse Hippocampus
56Fe Irradiation Alters Spine Density and Dendritic Complexity in the Mouse Hippocampus
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DOI：
10.1667/rr14103.1
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发表时间：
2015-12-01
影响因子：
3.4
通讯作者：
Fike, John R.
中科院分区：
文献类型：
--
作者：
Allen, Antino R.;Raber, Jacob;Fike, John R.
空间辐射环境的一个独特特征是存在高能带电粒子，这对在使命期间暴露于辐射中的空间飞行机组人员可能是非常危险的。与高LET辐射暴露相关的健康风险包括认知损伤。这种损伤的发病机制尚不清楚，但可能涉及树突结构的修饰和/或树突棘密度和形态的改变。在这项研究中，24只两个月大的C57 BL 6/J雄性小鼠用0.5戈伊56 Fe（600 MeV/n; n = 12）全身照射或假照射（n = 12）。辐射后三个月，测试动物的自发活动和习惯。在行为测试后，将动物安乐死，并将大脑快速冷冻。与假辐射小鼠相比，辐射小鼠在第一次被引入环境时移动较少，尽管它们在一天后重新暴露于环境时确实认识到了环境。暴露于56 Fe辐射显着损害了树突状结构，并降低了整个海马三突触网络的棘密度。据我们所知，这些数据代表了首次报道的证据表明，高LET辐射对海马学习和记忆相关的成熟神经元有有害影响。(C)2015年：辐射研究学会
A unique feature of the space radiation environment is the presence of high-energy charged particles, which can be significantly hazardous to space flight crews who are exposed during a mission. Health risks associated with high-LET radiation exposure include cognitive injury. The pathogenesis of this injury is unknown but may involve modifications to dendritic structure and/or alterations in dendritic spine density and morphology. In this study, 24 two-month-old C57BL6/J male mice were either whole-body irradiated with 0.5 Gy 56Fe (600 MeV/n; n = 12) or sham irradiated (n = 12). Three months postirradiation animals were tested for locomotor activity and habituation. After behavioral testing, animals were euthanized and the brains were flash frozen. Compared to sham-irradiated mice, irradiated mice moved less when first introduced to the environment, although they did recognize the environment when re-exposed to it one day later. Exposure to 56Fe radiation significantly compromised the dendritic architecture and reduced spine density throughout the hippocampal tri-synaptic network. To our knowledge, these data represents the first reported evidence that high-LET radiation has deleterious effects on mature neurons associated with hippocampal learning and memory. (C) 2015 by Radiation Research Society