Two sets of interacting collagens form functionally distinct substructures within a Caenorhabditis elegans extracellular matrix
Two sets of interacting collagens form functionally distinct substructures within a Caenorhabditis elegans extracellular matrix
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DOI：
10.1091/mbc.e02-08-0479
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发表时间：
2003-04-01
影响因子：
3.3
通讯作者：
Johnstone, IL
中科院分区：
文献类型：
--
作者：
McMahon, L;Muriel, JM;Johnstone, IL
胶原蛋白的普遍存在的特征是蛋白质相互作用，单体三聚化形成三螺旋，随后在成熟细胞外基质形成期间发生更高级的相互作用。秀丽隐杆线虫的表皮是一种复杂的细胞外基质，主要由表皮胶原蛋白组成，由一个类似于154个基因的家族编码。我们确定了两个离散的相互作用的胶原蛋白集，并表明它们形成功能不同的矩阵子结构。我们发现，突变或RNA介导的干扰属于一个相互作用的集合的胶原蛋白编码基因的影响，该集合的其他成员的组装，但不属于其他集。在角质层合成过程中，胶原蛋白基因表达在一个不同的时间序列，我们假设存在，以促进合作伙伴的发现和编码的胶原蛋白之间形成适当的相互作用。与这一假设相一致，我们发现，对于两个确定的相互作用的集合，每个集合的个体成员在时间上是共表达的，而这两个集合在矩阵合成过程中的表达相似于2 h。
A ubiquitous feature of collagens is protein interaction, the trimerization of monomers to form a triple helix followed by higher order interactions during the formation of the mature extracellular matrix. The Caenorhabditis elegans cuticle is a complex extracellular matrix consisting predominantly of cuticle collagens, which are encoded by a family of similar to154 genes. We identify two discrete interacting sets of collagens and show that they form functionally distinct matrix substructures. We show that mutation in or RNA-mediated interference of a gene encoding a collagen belonging to one interacting set affects the assembly of other members of that set, but not those belonging to the other set. During cuticle synthesis, the collagen genes are expressed in a distinct temporal series, which we hypothesize exists to facilitate partner finding and the formation of appropriate interactions between encoded collagens. Consistent with this hypothesis, we find for the two identified interacting sets that the individual members of each set are temporally coexpressed, whereas the two sets are expressed similar to2 h apart during matrix synthesis.