Endoplasmic reticulum stress induced by an ethanol extract of Coicis semen in Chang liver cells.
Endoplasmic reticulum stress induced by an ethanol extract of Coicis semen in Chang liver cells.
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DOI：
10.1186/s12906-018-2175-z
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发表时间：
2018-03-20
影响因子：
--
通讯作者：
Chun HS
中科院分区：
文献类型：
--
作者：
Kim HY;Song HN;Davaatseren M;Chang HJ;Chun HS
众所周知，内质网应激在代谢性疾病的发生发展中起着重要作用。特别是内质网应激诱导的细胞功能障碍在人类慢性疾病的发病机制中具有重要意义。本研究旨在研究薏苡仁醇提取物（CSE）和薏苡仁醇是否诱导Chang肝细胞的ER应激。将薏苡仁与95%乙醇以1：10（w/v）的比例混合并冷冻干燥。将Chang肝细胞接种到96孔板中，并用或不用CSE（100、200、300、500或1000 μg/mL）或薏苡醇（100、200、300、500、750或1000 μg/mL）处理。MTT法检测细胞活力。用qRT-PCR测定CSE和薏苡醇对ER应激标志物的基因表达的影响，并用蛋白质印迹法测定ER应激标志物的蛋白质水平的表达。CSE和薏苡仁醇的半数抑制浓度（IC 50）分别为250和350 μg/mL。CSE和薏苡仁醇以剂量依赖性方式增加BiP和CHOP的基因表达。此外，CSE和薏苡仁醇剂量依赖性地增加XBP 1的表达。CSE或薏苡仁醇可能对Chang肝细胞具有细胞毒性作用，并且可能通过激活正常肝细胞中的PERK和IRE 1途径诱导ER应激并刺激UPR。本文的在线版本（10.1186/s12906-018-2175-z）包含补充材料，可供授权用户使用。
It is well known that endoplasmic reticulum (ER) stress plays a huge role in development of metabolic diseases. Specially, ER stress-induced cellular dysfunction has a significant involvement in the pathogenesis of human chronic disorders. This study was designed to study to assess whether an ethanol extract of Coicis Semen (CSE) and coixol induces the ER stress in Chang liver cells. Coicis Semen was mixed with 95% ethanol at a ratio of 1:10 (w/v) and freeze dried. Chang liver cells were seeded to 96-well plates and treated with or without CSE (100, 200, 300, 500, or 1000 μg/mL) or coixol (100, 200, 300, 500, 750, or 1000 μg/mL). cell viability was analyzed with MTT assay. Effects of CSE and coixol on expression of the genes for ER stress markers were determined with qRT-PCR and the expression of the protein levels of ER stress markers were determined with western blotting. The concentration causing 50% inhibition (IC50) for CSE and coixol was 250 and 350 μg/mL, respectively. The CSE and coixol increased the gene expression of BiP and CHOP in a dose-dependent manner. Furthermore, CSE and coixol dose-dependently increased the the expression of XBP1. CSE or coixol may have cytotoxic effect to Chang liver cells and, may induce ER stress and stimulate the UPR via activation of the PERK and IRE1 pathways in normal liver cells. The online version of this article (10.1186/s12906-018-2175-z) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Hu LW;Yen JH;Shen YT;Wu KY;Wu MJ
通讯作者：
Wu MJ
DOI：
10.1016/j.bbrc.2012.11.058
发表时间：
2013-01-11
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
19.6
作者：
Balachandran, P;Wei, F;Pasco, DS
通讯作者：
Pasco, DS
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影响因子：
3.3
作者：
Haze, K;Yoshida, H;Mori, K
通讯作者：
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