Comparative biophysical study of adsorbed calf serum, fetal bovine serum and mussel adhesive protein films.
Comparative biophysical study of adsorbed calf serum, fetal bovine serum and mussel adhesive protein films.
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吸附小牛血清、胎牛血清和贻贝粘附蛋白膜的比较生物物理研究。
DOI：
10.1016/0142-9612(92)90185-q
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发表时间：
1992
期刊：
影响因子：
14
通讯作者：
Loomis,RE
中科院分区：
文献类型：
--
作者：
Olivieri,MP;Rittle,KH;Tweden,KS;Loomis,RE
不同浓度的不同血清和粘附剂通常用于增加细胞对基质的附着。使用椭圆偏振法、接触角分析和多重衰减内反射红外（MAIR-IR）光谱研究了这些表面改变材料的表面化学效应。具体地，使15%胎牛血清（FBS）、Ham’s F-12（含有10%FBS + 1%青霉素/链霉素）、10%小牛血清和贻贝粘附蛋白（MAP）吸附到相似和不同的表面上，然后进行比较。这些制剂中的每一种都能够以不同的所得表面能改变基质的表面化学性质。因此，为了了解界面效应，在所考虑的基质上表征适当浓度的血清是很重要的。
Varying concentrations of different sera and adhesive agents are routinely used to increase cellular attachment to substrata. The surface-chemical effects of some of these surface-altering materials have been examined using ellipsometry, contact angle analysis and multiple-attenuated internal reflection infrared (MAIR-IR) spectroscopy. Specifically, 15% fetal bovine serum (FBS), Ham's F-12 (containing 10% FBS + 1% penicillin/streptomycin), 10% calf serum and mussel adhesive protein (MAP) were allowed to adsorb on to similar and different surfaces and then compared. Each of these preparations is capable of altering the surface-chemical properties of substrata with varying resultant surface energies. It is therefore important to characterize serum in the proper concentrations on the substrata under consideration in order to understand the interfacial effects.