YAC contig organization and CpG island analysis in Xq28.
YAC contig organization and CpG island analysis in Xq28.
复制标题
Xq28 中的 YAC 重叠群组织和 CpG 岛分析。
DOI：
10.1006/geno.1994.1592
复制
发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Reinbold,R
中科院分区：
文献类型：
--
作者：
Palmieri,G;Romano,G;Ciccodicola,A;Casamassimi,A;Campanile,C;Esposito,T;Cappa,V;Lania,A;Johnson,S;Reinbold,R
将119个YAC组装成跨越约7.1Mb的Xq 28的6个重叠群。重叠群用65个STS和136个杂交探针格式化，并且足够广泛以通过公开的遗传连锁和体细胞杂交面板数据进行比对和定向。从整个地区选定的YAC映射与五个稀有切割酶的限制性内切酶推断的位置推定的CpG岛指示基因的位置; 48个这样的网站被确定的近重合的至少三个稀有切割位点。该分析确定了Xq 28的三个亚区：从Xq 27边界通过GABRA位点的中等GC和CpG岛含量的4 Mb;延伸到G6 PD基因的1.5 - 2 Mb，其被克隆且克隆较差，但含有高浓度的CpG岛和GC;在G6 PD和端粒之间约1.5Mb，其通常在CpG和GC水平上较低，包括显示出与Yq DNA广泛同源性的亚端粒DNA区域。
One hundred nineteen YACs were assembled into 6 contigs spanning about 7.1 Mb of Xq28. The contigs were formatted with 65 STSs and 136 hybridization probes and were extensive enough to be aligned and oriented by published genetic linkage and somatic cell hybrid panel data. Selected YACs from the entire region were mapped with five rare-cutter restriction enzymes to infer the position of putative CpG islands indicative of gene locations; 48 such sites were identified by the near-coincidence of at least three rare-cutter sites. The analysis defined three subregions of Xq28: 4 Mb of moderate GC and CpG island content from the Xq27 border through the GABRA locus; 1.5 to 2 Mb, extending to the G6PD gene, that is variably and poorly cloned, but contains a high concentration of CpG islands and GC; and about 1.5 Mb between G6PD and the telomere, which is generally low in CpG and GC levels, including a subtelomeric DNA region that shows extensive homology to Yq DNA.