Structure of the O-linked carbohydrate chains of porcine zona pellucida glycoproteins.
Structure of the O-linked carbohydrate chains of porcine zona pellucida glycoproteins.
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猪透明带糖蛋白的 O-连接碳水化合物链的结构。
DOI：
10.1111/j.1432-1033.1994.tb18762.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
J. Vliegenthart
中科院分区：
文献类型：
--
作者：
C. Hokke;J. B. Damm;B. Penninkhof;R. Aitken;J. Kamerling;J. Vliegenthart
用肽-N4-（N-乙酰基-β-葡糖胺基）天冬酰胺酰胺酶F消化猪透明质酸糖蛋白的N-连接糖链，随后用Bio-Gel P-100上的凝胶渗透色谱将其与O-糖蛋白分离。通过碱性硼氢化物处理从O-糖蛋白释放O-连接的糖链。通过组合色谱技术（包括Bio-Gel P-4和P-6上的凝胶渗透色谱、Mono Q上的阴离子交换FPLC和CarboPac PA-1上的高pH阴离子交换色谱）实现O-连接寡糖糖醇的极不均匀混合物的分馏。通过一维和二维1H-NMR光谱确定了32个O-聚糖的一级结构。分析的化合物的主要部分含有结构元件Gal β 1-4GlcNAc β 1 -3Gal β 1 - 3GalNAc-醇、Gal β 1-4（6SO 4-）GlcNAc和α 2-3-连接的Neu 5Gc或Neu 5Ac的组合。该系列化合物具有以下结构，其中n = 0至> 6：[Neu 5Gc/Ac α 2 -3]0-1[Gal β 1-4（6SO 4-）GlcNAc β 1-3]nGal β 1-4GlcNAc β 1 -3Gal β 1 - 3GalNAc-醇。此外，鉴定了较小的化合物，其中Gal β 1- 3GalNAc-醇核心被与GalNAc-醇2-6-连接的Neu 5Gc/Ac α和/或与Gal 2-3-连接的Neu 5Gc/Ac α取代。此外，获得了低聚糖，其中6-O-硫酸化GlcNAc残基的分布不同于上述一般结构中的分布，并且低聚糖的小部分具有GlcNAc β 1- 3GalNAc-醇核心结构。对N-和O-聚糖池的内切-β-半乳糖苷酶活性的分析表明，这两种类型的寡糖含有定性相似的聚（N-乙酰乳糖胺）链。在N-连接的碳水化合物链的情况下，发生核心结构的多个分支，导致比O-连接的碳水化合物链观察到的更大的异质性。
The N-linked carbohydrate chains of porcine zona pellucida glycoproteins were released by digestion with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F and subsequently separated from the O-glycoprotein by gel-permeation chromatography on Bio-Gel P-100. The O-linked carbohydrate chains were released from the O-glycoprotein by alkaline borohydride treatment. Fractionation of the extremely heterogeneous mixture of O-linked oligosaccharide alditols was achieved by a combination of chromatographic techniques comprising gel-permeation chromatography on Bio-Gel P-4 and P-6, anion-exchange FPLC on Mono Q, and high-pH anion-exchange chromatography on CarboPac PA-1. The primary structures of 32 O-glycans were determined by one- and two-dimensional 1H-NMR spectroscopy. The major part of the analyzed compounds contain a combination of the structural elements Gal beta 1-4GlcNAc beta 1-3Gal beta 1-3GalNAc-ol, Gal beta 1-4(6SO4-)GlcNAc, and alpha 2-3-linked Neu5Gc or Neu5Ac. This series of compounds has the following structure, where n = 0 to > 6: [Neu5Gc/Ac alpha2-3]0-1[Gal beta 1-4(6SO4-)GlcNAc beta 1-3]nGal beta 1-4GlcNAc beta 1-3Gal Beta 1-3GalNAc-ol. In addition, smaller compounds were identified in which the Gal beta 1-3GalNAc-ol core is substituted by Neu5Gc/Ac alpha 2-6-linked to GalNAc-ol and/or Neu5Gc/Ac alpha 2-3-linked to Gal. Furthermore, oligosaccharides were obtained in which the distribution of 6-O-sulfated GlcNAc residues differs from that in the above-mentioned general structure, and a small portion of the oligosaccharides has the GlcNAc beta 1-3GalNAc-ol core structure. Analysis of the endo-beta-galactosidase digests of pools of N- and O-glycans indicated that the two types of oligosaccharides contain qualitatively similar poly(N-acetyllactosamine) chains. In the case of the N-linked carbohydrate chains, multiple branching of the core structures occurs, resulting in an even larger heterogeneity than observed for the O-linked carbohydrate chains.
DOI：
10.1016/s0021-9258(18)35777-6
发表时间：
1992-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Maemura;M. Fukuda
通讯作者：
K. Maemura;M. Fukuda
大鼠透明带糖缀合物上存在的寡糖链的定性表征。
影响因子：
3.6
作者：
Araki,Y;Orgebin-Crist,MC;Tulsiani,DR
通讯作者：
Tulsiani,DR
从正常粒细胞、慢性粒细胞白血病细胞和急性粒细胞白血病细胞中分离出的 O-连接寡糖的结构。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fukuda,M;Carlsson,SR;Klock,JC;Dell,A
通讯作者：
Dell,A