Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification
Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification
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DOI：
10.1007/s00253-010-2484-8
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发表时间：
2010-06-01
影响因子：
5
通讯作者：
Yang, Jianming
中科院分区：
文献类型：
--
作者：
Li, Qiang;Jiang, Xinglin;Yang, Jianming
离子液体预处理木质纤维素材料是一种很有前途的生物质转化为可再生生物燃料的方法。因此，需要对环境友好型离子液体进行更深入的研究，探索前处理的绿色路线。本研究选择IL 1-甲基-3-甲基咪唑亚磷酸二甲酯（[Mmim]咪唑）作为预处理玉米芯的环境友好溶剂，考虑到其对木质纤维素溶解性和纤维素酶活性的生物相容性。预处理/糖化过程和[Mmim]底物参与的原位糖化过程在玉米芯生物转化为糖中有效地进行，并且获得大于70%的糖化率。此外，使用不透明红球菌菌株ACCC 41043（R.不透明）。培养30小时后，获得高脂质产量41-43%的细胞干物质。GC/MS分析表明，R. Opacus主要含有长链脂肪酸，具有四种主要成分/油酸、硬脂酸、棕榈酸、棕榈油酸，它们是生物柴油的良好候选物。这说明IL [Mmim]DMP预处理的玉米芯不会对R.的糖化、细胞生长和脂质积累产生负面影响。不透明综上所述，IL [Mmim]-N显示出作为纤维素材料的绿色预处理溶剂的前景。
Ionic liquid (IL) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. More in-depth research involving environment-friendly IL is much needed to explore pretreatment green route. In our case, IL 1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) was chosen as an environment-friendly solvent to pretreat corn cob in view of its biocompatibility with both lignocellulose solubility and cellulase activity. The pretreatment/saccharification process and in situ saccharification process involving [Mmim]DMP were efficiently performed in bioconversion of corn cob to sugars, and more than 70% saccharification rates were obtained. Furthermore, the fermentability of reducing sugars obtained from the hydrolyzates was evaluated using Rhodococcus opacus strain ACCC41043 (R. opacus). High lipid production 41-43% of cell dry matter was obtained after 30 h of cultivation. GC/MS analysis indicated that lipids from R. opacus contained mainly long-chain fatty acids with four major constituent/oleic acid, stearic acid, palmitic acid, palmitoleic acid which are good candidates for biodiesel. These elucidated that corn cob pretreated by IL [Mmim]DMP did not bring negative effects on saccharification, cell growth, and accumulation of lipid of R. opacus. In conclusion, the IL [Mmim]DMP shows promise as green pretreatment solvent for cellulosic materials.