Spatio-temporal expression of Pbx3 during mouse organogenesis
Spatio-temporal expression of Pbx3 during mouse organogenesis
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DOI：
10.1016/j.modgep.2005.12.002
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发表时间：
2006-10-01
影响因子：
1.2
通讯作者：
Selleri, Licia
中科院分区：
文献类型：
--
作者：
Di Giacomo, Giuseppina;Koss, Matthew;Selleri, Licia
Pbx3是三氨基酸环延伸(TALE)类同源结构域转录因子的PBX家族成员。这些转录因子通过形成异寡聚体DNA结合复合体，参与多种细胞类型的发育和转录基因调控。研究发现，PBX3在发育中的中枢神经系统(CNS)中高水平表达，包括与呼吸控制有关的延髓区域。此外，据报道，Pbx3缺陷小鼠发育到足月，但在出生后几个小时内死于中枢性呼吸衰竭。在这项研究中，我们研究了Pbx3在中枢神经系统以外的许多组织和器官系统器官发生过程中的表达模式，作为了解Pbx3在脊椎动物发育过程中与其他PBX家族成员潜在重叠功能的第一步。我们对孕9天至16天的小鼠整体胚胎和切片胚胎进行了原位杂交。在器官发生的早期，直到E12.5，Pbx3主要在胚胎头部、前肢和横隔中表达，不像Pbx1和Pbx2表达更广泛。相反，在器官发生的后期，Pbx3的表达在整个发育中的胚胎中变得更加广泛地被检测到。上皮和间质组织以及中枢神经系统是Pbx3表达的主要部位。肠道神经系统也表达高水平的Pbx3，特别是在奥尔巴赫肌间神经丛的神经节细胞中，也表达Dlx2和Notch1。软骨也是Pbx3表达的部位。有趣的是，像Pbx1一样，Pbx3在增殖的软骨细胞中高表达，但随着软骨细胞在软骨内成骨过程中变得肥大而丢失。最后，在肢体发育早期，Pbx3仅在前肢芽中表达，而在后肢芽中不表达。这一发现导致我们将Pbx3添加到早期前肢特异分子标记的稀疏列表中。爱思唯尔出版公司(Elsevier B.V.)
Pbx3 is a member of the Pbx family of TALE (three amino acid loop extension) class homeodomain transcription factors. These transcription factors are implicated in developmental and transcriptional gene regulation in numerous cell types through their abilities to form hetero-oligomeric DNA-binding complexes. Pbx3 was found to be expressed at high levels in the developing central nervous system (CNS), including a region of the medulla oblongata which is implicated in the control of respiration. Furthermore, as reported, Pbx3-deficient mice develop to term but die within a few hours of birth from central respiratory failure. In this study, we have characterized Pbx3 expression patterns during organogenesis in numerous tissues and organ systems other than the CNS, as a first step toward understanding the potentially overlapping functions of Pbx3 with other Pbx family members during vertebrate development. We have performed in situ hybridization on whole mount and sectioned mouse embryos from gestational day (E) 9 to E16.5. During early organogenesis, until E12.5, Pbx3 expression is found mostly in the embryonic head, forelimbs, and septum transversum, unlike Pbx1 and Pbx2 expression which is more widespread. Conversely, later in organogenesis, Pbx3 expression becomes more widely detectable throughout the developing embryo. Epithelial and mesenchymal tissues, as well as the CNS, represent major sites of Pbx3 expression. The enteric nervous system also expresses high levels of Pbx3, distinctively in the cells of the ganglia of Auerbach's myenteric nerve plexus, that also express Dlx2 and Notch1. Cartilage is also a site of Pbx3 expression. Interestingly, like Pbx1, Pbx3 is highly expressed in proliferating chondrocytes but is lost as chondrocytes become hypertrophic during endochondral ossification. Finally, Pbx3 is expressed only in the forelimb buds during early limb development, while the hindlimb bud is devoid of Pbx3. This finding leads us to add Pbx3 to the sparse list of early forelimb-specific molecular markers. Published by Elsevier B.V.