Comparative characterization of aluminum responsive transcriptome in Arabidopsis roots: comparison with other rhizotoxic ions at different stress intensities
Comparative characterization of aluminum responsive transcriptome in Arabidopsis roots: comparison with other rhizotoxic ions at different stress intensities
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DOI：
10.1080/00380768.2018.1454253
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发表时间：
2018-03
影响因子：
2
通讯作者：
Kazutaka Kusunoki;Yasufumi Kobayashi;Yuriko Kobayashi;H. Koyama
中科院分区：
文献类型：
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作者：
Kazutaka Kusunoki;Yasufumi Kobayashi;Yuriko Kobayashi;H. Koyama
摘要在非生物胁迫条件下，植物会主动改变转录组，以激活胁迫耐受机制或调整生物学过程以适应胁迫。不同胁迫和胁迫强度下的转录组数据的比较分析有助于了解植物在胁迫条件下如何平衡生长和存活。在这项研究中，拟南芥根的转录组响应于四个根毒性胁迫，即铝（Al 3+），镉（Cd 2+），铜（Cu 2+）离子和氯化钠（NaCl），在轻度胁迫（根伸长抑制50%与对照相比）和严重胁迫（根伸长抑制>90%）。只有Al处理在胁迫强度之间显示出相似的转录组响应。铝特异性上调表达的基因包括已知的耐铝基因和敏感质子1（STOP 1）的下游基因，如铝激活的苹果酸转运蛋白1（ALUMINUM ACTIVATED MALATE TRANSPORTER1）和苹果酸酶，表明STOP 1调控的铝防御机制可能在更广泛的铝胁迫强度下被激活。硫代谢途径仅在严重铝胁迫下才被激活，表明硫代谢的快速激活是严重铝胁迫初期根系细胞存活的关键。在重度和轻度胁迫下，Cd和Cu处理均上调了大量与活性氧产生相关的基因，但不同胁迫程度下基因组的成员有所不同。结果表明，Cd和Cu胁迫诱导的转录反应是相同的，但这些反应可能与胁迫程度有关。与其他胁迫因子相比，对轻度NaCl胁迫的转录响应相对较小，这表明在拟南芥中，除了快速转录组学响应之外，还涉及对NaCl胁迫的响应。铝胁迫与其他胁迫因子的比较表明，STOP 1调控系统和中枢代谢途径导致了不同强度铝胁迫下转录反应的相似性。这些结果表明，转录调控的耐铝系统对不同胁迫强度的鲁棒性对植物根系在铝胁迫下的生长和存活具有重要意义。
ABSTRACT Under abiotic stress conditions, plants actively modify the transcriptome either for activation of stress tolerance mechanisms or adjustment of biological processes for adaptation to the stress. Comparative analysis of transcriptome data under different stressors and stress intensities is useful to understand how plants balance growth and survival under stress conditions. In this study, the transcriptome of Arabidopsis roots was investigated in response to four rhizotoxic stressors, namely aluminum (Al3+), cadmium (Cd2+), and copper (Cu2+) ions and sodium chloride (NaCl), under mild stress (50% inhibition of root elongation compared with the control) and severe stress (>90% inhibition of root elongation). Only Al treatment showed similar transcriptome responses between the stress intensities. The shared Al-specific up-regulated genes included known Al-tolerance genes and downstream genes of SENSITIVE TO PROTON1 (STOP1) such as ALUMINUM ACTIVATED MALATE TRANSPORTER1 and malic enzymes, suggesting that Al-defense mechanisms regulated by STOP1 might be activated over a broader range of Al stress intensities. The sulfur metabolism pathway was activated only under severe Al stress, suggesting that rapid activation of sulfur metabolism is crucial for survival of root cells in the early stage of severe Al stress. A large number of genes associated with the response mechanism to reactive oxygen species production were up-regulated by Cd and Cu treatment under both severe and mild stress, but the members of the genes sets differed between the stress severities. The results indicated that Cd and Cu stress trigger common transcriptional responses, but the responses are likely associated with the stress severity. The transcriptional response to mild NaCl stress was relatively smaller compared with those to the other stressors, suggesting that in Arabidopsis different mechanisms other than rapid transcriptomic responses are involved in the response to NaCl stress. Comparison between Al and the other stressors indicated that the STOP1 regulation system and central metabolic pathways contribute to the similarity of the transcriptional response to different intensities of Al stress. The results suggest that robustness of transcriptionally regulated Al tolerance systems to different stress intensities is important for both of growth and survival in plant roots under Al stress.