Cloning of a salivary gland metalloprotease and characterization of gelatinase and fibrin(ogen)lytic activities in the saliva of the Lyme disease tick vector Ixodes scapularis
Cloning of a salivary gland metalloprotease and characterization of gelatinase and fibrin(ogen)lytic activities in the saliva of the Lyme disease tick vector Ixodes scapularis
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DOI：
10.1016/s0006-291x(03)00857-x
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发表时间：
2003-06-13
影响因子：
3.1
通讯作者：
Ribeiro, JMC
中科院分区：
文献类型：
--
作者：
Francischetti, IMB;Mather, TN;Ribeiro, JMC
本文报道了蜱唾液腺cDNA全长序列，编码一种类似于replysin家族金属蛋白酶（NIP）的蛋白。肩突硬蜱NIP是一种488个氨基酸（aa）的蛋白质，其含有前酶和酶原结构域、金属蛋白酶共有的锌结合基序HExxHxxGxxH和富含半胱氨酸的区域。此外，预测的I.通过Edman降解PVDF转移的SDS/PAGE分离的蜱唾液蛋白发现肩胛肌MP，表明这些推定的酶是分泌的。此外，唾液对明胶、纤维蛋白（原）具有金属依赖性蛋白水解活性。和纤连蛋白，而不是胶原蛋白或层粘连蛋白。因此，肩胛乳杆菌唾液具有相当特异的金属蛋白酶，类似于蛇毒的出血性蛋白酶。这是第一次描述这种活动在蜱唾液和蜱喂养和疏螺旋体传播中的作用进行了讨论。(C)2003 Elsevier Science（美国）。All rights reserved.
The full-length sequence of tick salivary gland cDNA coding for a protein similar to metalloproteases (NIP) of the reprolysin family is reported. The Ixodes scapularis NIP is a 488 amino acid (aa) protein containing pre- and pro-enzyme domains, the zinc-binding motif HExxHxxGxxH common to metalloproteases, and a cysteine-rich region. In addition, the predicted amino-terminal sequences of I. scapularis MPs were found by Edman degradation of PVDF-transferred SDS/PAGE-separated tick saliva proteins, indicating that these putative enzymes are secreted. Furthermore, saliva has a metal-dependent proteolytic activity towards gelatin, fibrin(ogen). and fibronectin, but not collagen or laminin. Accordingly, L scapularis saliva has a rather specific metalloprotease similar to the hemorrhagic proteases of snake venoms. This is the first description of such activity in tick saliva and its role in tick feeding and Borrelia transmission is discussed. (C) 2003 Elsevier Science (USA). All rights reserved.