EFFECT OF JUVENILE-HORMONE TREATMENT ON CASTE DIFFERENTIATION IN HONEYBEE, APIS-MELLIFERA
EFFECT OF JUVENILE-HORMONE TREATMENT ON CASTE DIFFERENTIATION IN HONEYBEE, APIS-MELLIFERA
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DOI：
10.1016/0022-1910(74)90225-x
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发表时间：
1974-01-01
影响因子：
2.2
通讯作者：
RAO, PJ
中科院分区：
文献类型：
--
作者：
REMBOLD, H;CZOPPELT, C;RAO, PJ
将合成保幼激素（甲基反式，反式，顺式-10-环氧-7-乙基-3，11-二甲基-2，6-十三碳二烯酸酯，1 μg/μl丙酮/动物）（JH）局部施用于蜂巢中的2至3日龄工蜂幼虫。80%的幼虫处理的幼虫从他们的育雏室化蛹前。42名成年人中只有1人表现出跨种姓的特征。对照幼虫（1 μl丙酮）50%发育成成虫，全部为工蜂，在体外条件下，用JH局部涂抹和取食皇家浆后，存活率高（达85%），但高达67%的蜂王和44%的工蜂出现具有体细胞突变特征的眼畸形。激素处理的结果是，纺绩幼虫形成更坚固的网，滞育缩短1至2天，下颚、腿和腹部的形状不寻常。用0.1 μg激素代替1 μg激素或在食物中添加激素（2 μg/g皇家浆）后，效果不明显。处理2至3日龄的工蜂幼虫，随后用皇家浆饲养，然后是从蜂王和工蜂到中间种性的种姓分化。无论如何，在保幼激素处理后，更多的蜂王发育。蜂王决定子，部分纯化的皇家果冻，诱导从工人到蜂王分化的浓度依赖性转变。三倍的自然决定因素浓度的皇家果冻的结果在一个几乎排他性的（98%）女王形成从2至3日龄的工人幼虫。在这种直接的影响相反，JH的影响被解释为间接的形态发生的影响，不直接与蜜蜂种姓分化。
Synthetic juvenile hormone (methyltrans,trans,cis-10-epoxy-7-ethyl-3,11-dimethyl-2,6-tridecadienoate, 1 μg/μl acetone per animal) (JH) was topically applied to 2- to 3-day-old worker honeybee larvae in the hive. Eighty per cent of the hormone-treated larvae were removed from their brood cell before pupation. Only 1 out of 42 adults showed characteristics of an intercaste. Fifty per cent of the control larvae (1 μl acetone) developed to adults, all of which were workers.After topical application of JH and feeding on royal jelly underin vitroconditions, the rate of survival is high (up to 85 per cent adults), but up to 67 per cent of queens and 44 per cent of workers exhibit eye malformations with characteristics of somatic mutation. Formation of a more solid web by the spinning larvae, shortening of the diapause by 1 to 2 days, and unusual shapes of mandibles, legs, and abdomen are a consequence of hormone treatment. The effects are less marked after application of 0·1 instead of 1 μg hormone or after its addition to the food (2 μg/g royal jelly). Treatment of the 2- to 3-day-old worker larvae and subsequent rearing on royal jelly is followed by a shift in caste differentiation from queens and workers to intercastes. In no case, are more queens developed after juvenile hormone treatment.Queen bee determinator, partially purified from royal jelly, induces a concentration-dependent shift from workers to queen differentiation. A threefold increase in the natural determinator concentration of royal jelly results in an almost exclusive (98 per cent) queen formation from 2- to 3-day-old worker larvae. In contrast to this direct effect, the influence of JH is explained as an indirect morphogenetic effect not directly coupled with honeybee caste differentiation.