Markers of thromboembolization in a bovine ex vivo left ventricular assist device model.
Markers of thromboembolization in a bovine ex vivo left ventricular assist device model.
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DOI：
10.1097/00002480-199407000-00070
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发表时间：
1994-07-01
期刊：
影响因子：
--
通讯作者：
Olsen, D B
中科院分区：
文献类型：
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作者：
Solen, K A;Mohammad, S F;Olsen, D B
用体外体外左心室辅助装置(LVAD)模型研究了小牛血液微栓子(BME)的产生。8头小牛每头接受一系列的三个LVAD，每个LVAD操作24小时。用激光(624 nm和828 nm)光散射微栓子检测系统(LSMD)通过LVAD流出管直接测量血液微栓子，并对流出管中的血样进行恒压过滤(CPF)。同时测定血液学参数。左前降支摘除后，用极坐标标测评价瓣膜周围血栓。平均LSMD和CPF结果相关。例如，根据LSMD和CPF的结果，在一个三头小牛的系列中，一个脑室比另一个脑室表现出显著更大的血栓形成。在一系列的五头小牛中，一头小牛出现了异常高的活化凝血活酶时间(APTT)，即使在没有肝素的情况下。在这头犊牛的三个脑室中，有两个的微栓子浓度(CPF)、凝血因子XII活性、纤维蛋白降解产物(FDP)水平和累积血栓显著低于其他犊牛。这头犊牛的全血粘度(WBV230 S-1)也降到了比其他犊牛更低的水平。
The production of blood microemboli (BME) was studied using an ex vivo exteriorized left ventricular assist device (LVAD) model in calves. Each of eight calves received a series of three LVADs, each operating for 24 hr. Blood microemboli were measured directly by a laser (624 nm and 828 nm) light scattering microemboli detection (LSMD) system through the LVAD outflow cannula and by constant pressure filtration (CPF) of blood samples from the LVAD outflow cannula. Hematologic parameters were also measured. After LVAD removal, perivalvular thrombi were evaluated using polar coordinate mapping. The average LSMD and CPF results correlated. For example, in one series of three calves, one ventricle exhibited significantly greater thrombogenesis than did the other ventricles, as indicated by both the LSMD and CPF results. In a series of five calves, one calf developed an abnormally high activated thromboplastin time (APTT), even in the absence of heparin. For two of the three ventricles tested in that calf, microemboli concentration (CPF), Factor XII activity, level of fibrin degradation products (FDP), and accumulated thrombus were significantly lower than for the other calves. The whole blood viscosity (WBV, at 230 s-1) in this calf also decreased to lower values than were seen with the other calves.