Augmentation of TH-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signaling
Augmentation of TH-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signaling
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DOI：
10.1016/j.bbrc.2004.11.119
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发表时间：
2005-01-28
影响因子：
3.1
通讯作者：
Saito, T
中科院分区：
文献类型：
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作者：
Shimosato, T;Kitazawa, H;Saito, T
Toll 样受体 9 在抗原呈递细胞表面表达，最近在细胞质滤泡中被发现，可识别细菌 CpG 寡脱氧核苷酸 (ODN)，从而诱导有效的免疫反应。然而，在我们之前的研究中，我们发现TLR9不仅可以识别CpG ODN，还可以识别非CpG ODN，例如AT ODN。因此，在本研究中，为了探讨这种可能性，我们通过猪 TLR9 转染子的实时定量 PCR 分析和 ELISA 阐明了 AT ODN 对 TLR9 诱导 T-H-1、T-H-2 型细胞因子的影响。结果表明，与未暴露的对照相比，AT ODN 强烈诱导 T-H-1 型细胞因子，如白细胞介素 (IL)-12p70 和干扰素 (IFN)-γ，而 T-H-2 型细胞因子则不被诱导。这些结果表明AT ODN可以增强T-H-1免疫反应，在预防过敏反应中发挥重要作用。此外，猪 TLR9 转染子通过 TLR9 信号传导检测 T-H-1、T-H-2 型细胞因子诱导，证明了其可用于评估细菌 DNA 的免疫刺激作用。 (C) 2004 Elsevier Inc. 保留所有权利。
Toll-like receptor 9, which is expressed on the surface of antigen presenting cells and which was recently identified in the cytoplasmic follicle, recognizes bacterial CpG oligodeoxynucleotides (ODNs), resulting in the induction of a potent immune response. However, in our previous study, we found that TLR9 potentially recognizes not only CpG ODN but also non-CpG ODN such as AT ODN. Therefore, in the present study, to investigate this possibility, we elucidated the effects of AT ODN on T-H-1, T-H-2 type cytokine induction via TLR9 by real-time quantitative PCR analysis and ELISA of the swine TLR9 transfectant. The results demonstrated that the T-H-1 type cytokines such as interleukin (IL)-12p70 and interferon (IFN)-gamma were strongly induced by AT ODN compared to the unexposed controls, while T-H-2 type cytokines were not induced. These results indicate that the AT ODN can augment the T-H-1 immune response, which plays an important role in prevention of allergic responses. Moreover, the swine TLR9 transfectant demonstrated its usefulness for evaluation of immunostimulation by bacterial DNA through the detection of T-H-1, T-H-2 type cytokine induction via TLR9 signaling. (C) 2004 Elsevier Inc. All rights reserved.