RBCs as microlenses: wavefront analysis and applications
RBCs as microlenses: wavefront analysis and applications
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DOI：
10.1117/12.2272042
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
F. Merola;Á. Barroso;L. Miccio;P. Memmolo;M. Mugnano;P. Ferraro;C. Denz
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文献类型：
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作者：
F. Merola;Á. Barroso;L. Miccio;P. Memmolo;M. Mugnano;P. Ferraro;C. Denz
发展最近发现的红细胞作为微透镜的概念，我们展示了在波前分析和诊断中的进一步应用。建立了红细胞形态与其折射光学特性之间的相关性。事实上，与健康RBC形态的任何偏差都可以被视为穿过细胞的光学波前中的额外像差。通过这个概念，红细胞焦点的准确定位在血液疾病识别中可以变得非常有用。此外，通过Zernike多项式将RBC建模为生物透镜，可以识别一系列能够识别RBC形状的正交参数。主要的改进涉及联合收割机的可能性，因为它们的独立性，相反，标准的基于图像的分析，其中形态因素是相互依赖的。我们研究了两种不同渗透压条件下，即圆盘细胞和球形细胞的样品随着时间的推移，这样的焦点的三维定位。最后，Zernike多项式波前分析使我们能够研究红细胞在光诱导机械应力下的光学行为。详细的波前分析提供了全面的信息所引起的变形使用光镊。由于干涉仪工具的高灵敏度，这可以通过检查光学参数而不是直接但具有低分辨率的应变分析来打开分析细胞弹性的新路线。
Developing the recently discovered concept of RBCs as microlenses, we demonstrate further applications in wavefront analysis and diagnostics. Correlation between RBC’s morphology and its behavior as a refractive optical element has been established. In fact, any deviation from the healthy RBC morphology can be seen as additional aberration in the optical wavefront passing through the cell. By this concept, accurate localization of focal spots of RBCs can become very useful in blood disorders identification. Moreover, By modelling RBC as bio-lenses through Zernike polynomials it is possible to identify a series of orthogonal parameters able to recognise RBC shapes. The main improvement concerns the possibility to combine such parameters because of their independence conversely to standard image-based analysis where morphological factors are dependent each-others. We investigate the three-dimensional positioning of such focal spots over time for samples with two different osmolarity conditions, i.e. discocytes and spherocytes. Finally, Zernike polynomials wavefront analysis allows us to study the optical behavior of RBCs under an optically-induced mechanical stress. Detailed wavefront analysis provides comprehensive information about the aberrations induced by the deformation obtained using optical tweezers. This could open new routes for analyzing cell elasticity by examining optical parameters instead of direct but with low resolution strain analysis, thanks to the high sensitivity of the interferometric tool.