Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken
Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken
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DOI：
10.1016/j.gep.2007.11.001
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发表时间：
2008-02-01
影响因子：
1.2
通讯作者：
Asahara, Hiroshi
中科院分区：
文献类型：
--
作者：
Yokoyama, Shigetoshi;Hashimoto, Megumi;Asahara, Hiroshi
秀丽线虫的异时基因Lin-28调节线虫主干的发育时间。我们报道了LIN-28同源物在小鼠和鸡胚胎中的动态表达模式。整装原位杂交显示了LIN-28在发育中的小鼠和雏鸡肢芽中的特异和有趣的表达模式。小鼠LIN-28在E9.5的前肢和后肢均有表达，但在E10.5的前肢表达消失，在E11.5的前肢和后肢表达消失。在第15/16期首次在肢体原基中检测到的鸡LIN-28，也随着阶段的进行而下调。小鼠和鸡LIN-28基因的氨基酸序列高度保守，并且LIN-28在小鼠和鸡肢体发育过程中的表达模式相似，表明该异时基因在脊椎动物肢体发育过程中也是保守的。(C)2007 Elsevier B.V.保留所有权利。
The Caenorhabditis elegans heterochronic gene lin-28 regulates developmental timing in the nematode trunk. We report the dynamic expression patterns of Lin-28 homologues in mouse and chick embryos. Whole mount in situ hybridization revealed specific and intriguing expression patterns of Lin-28 in the developing mouse and chick limb bud. Mouse Lin-28 expression was detected in both the forelimb and hindlimb at E9.5, but disappeared from the forelimb at E10.5, and finally from the forelimb, and hindlimb at E11.5. Chicken Lin-28, which was first detected in the limb primordium at stage 15/16, was also downregulated as the stage proceeded. The amino acid sequences of mouse and chicken Lin-28 genes are highly conserved and the similar expression patterns of Lin-28 during limb development in mouse and chicken suggest that this heterochronic gene is also conserved during vertebrate limb development. (c) 2007 Elsevier B.V. All rights reserved.