Biophysical investigation of pulmonary surfactant surface properties upon contact with polymeric nanoparticles in vitro
Biophysical investigation of pulmonary surfactant surface properties upon contact with polymeric nanoparticles in vitro
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DOI：
10.1016/j.nano.2010.10.007
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发表时间：
2011-06-01
影响因子：
5.4
通讯作者：
Gessler, Tobias
中科院分区：
文献类型：
--
作者：
Beck-Broichsitter, Moritz;Ruppert, Clemens;Gessler, Tobias
纳米颗粒药物载体已被提议用于药物向肺部的靶向和受控释放。然而，吸入颗粒可能会对肺表面活性物质的生物物理特性产生不利影响。本研究探讨了具有独特物理化学性质的聚合物纳米颗粒对肺表面活性剂的吸附和动态表面张力降低特性的影响。纳米颗粒的平均尺寸为 100 nm，尺寸分布窄。尽管聚（苯乙烯）和聚（D，L-丙交酯-乙交酯）纳米颗粒对肺表面活性剂的生物物理学具有剂量依赖性影响，但由聚（甲基丙烯酸丁酯-共聚（2-二甲氨基乙基）甲基丙烯酸酯-共聚甲基丙烯酸甲酯）制成的带正电纳米颗粒没有显示出影响。这种行为归因于 zeta 电位和表面疏水性的差异，这反过来又涉及带正电的表面活性剂蛋白对纳米颗粒的吸附模式的改变。这项研究表明，聚合物纳米颗粒不会显着影响肺表面活性剂的生物物理性质，并且可能成为呼吸系统疾病吸入治疗的可行药物输送载体。来自临床编辑：吸入纳米颗粒药物载体可能会对肺表面活性剂的生物物理性质产生不利影响。在本研究中，从这个角度表征了聚合物纳米颗粒的影响，得出的结论是聚合物纳米颗粒不会显着影响肺表面活性剂的生物物理性质，并且可能是用于吸入治疗的可行的药物递送载体。 (C) 2011 Elsevier Inc. 保留所有权利。
Nanoparticulate drug carriers have been proposed for the targeted and controlled release of pharmaceuticals to the lung. However, inhaled particles may adversely affect the biophysical properties of pulmonary surfactant. This study examines the influence of polymeric nanoparticles with distinct physicochemical properties on the adsorption and dynamic surface tension lowering properties of pulmonary surfactant. Nanoparticles had a mean size of 100 nm with narrow size distributions. Although poly(styrene) and poly(D, L-lactide-co-glycolide) nanoparticles revealed a dose-dependent influence on biophysics of pulmonary surfactant, positively-charged nanoparticles made from poly(butyl methacrylate-co-(2-dimethylaminoethyl) methacrylate-co-methyl methacrylate) showed no effect. This behavior is attributed to the differences in zeta-potential and surface hydrophobicity, which in turn involves an altered adsorption pattern of the positively charged surfactant proteins to the nanoparticles. This study suggests that polymeric nanoparticles do not substantially affect the biophysical properties of pulmonary surfactant and may be a viable drug-delivery vehicle for the inhalative treatment of respiratory diseases.From the Clinical Editor: Inhaled nanoparticulate drug carriers may adversely affect the biophysical properties of pulmonary surfactant. In this study the influence of polymeric nanoparticles was characterized from this standpoint, with the conclusion that polymeric nanoparticles do not substantially affect the biophysical properties of pulmonary surfactant and may be viable drug-delivery vehicles for inhalational treatment. (C) 2011 Elsevier Inc. All rights reserved.