Bystander effect on cell growth stimulation in neoplastic HSGc cells induced by heavy-ion irradiation
Bystander effect on cell growth stimulation in neoplastic HSGc cells induced by heavy-ion irradiation
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DOI：
10.1007/s00411-003-0202-y
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发表时间：
2003-10-01
影响因子：
1.7
通讯作者：
Furusawa, Y
中科院分区：
文献类型：
--
作者：
Shao, CL;Aoki, M;Furusawa, Y
用290 MeV/u不同线能量转移（LET）的碳束照射人涎腺肿瘤（HSGc）细胞，并与未照射的HSGc细胞共培养，观察旁观者效应。结果表明，未照射的受体细胞的接种效率和增殖能力提高，这些提高与LET以及辐射剂量有关。HSGc细胞暴露于高LET和高剂量比暴露于低LET和低剂量更有效地提高未照射细胞的接种效率和增殖。然而，当PTIO，一氧化氮（NO）的特异性清除剂，存在于共培养基中，未照射受体的细胞生长能力降低到对照水平，表明NO参与旁观者反应。亚硝酸盐作为NO的氧化产物，在共培养液中检测到，其浓度依赖于LET和辐照剂量。使用NO-发生器NO2/NO，证实了低浓度的NO确实增强了细胞增殖。因此，NO在介质介导的旁观者效应中起重要作用。
The bystander effect on unirradiated neoplastic human salivary gland (HSGc) cells was investigated by co-culturing them with HSGc cells that had been irradiated with 290 MeV/u carbon beams of different linear energy transfer (LET) values. It was found that the plating efficiency and proliferation of the unirradiated recipient cells were increased and that these increases were related to the LET as well as the radiation dose. Exposure of HSGc cells to higher LET and higher dose was much more effective in enhancing the plating efficiency and proliferation of the unirradiated cells than exposure to lower LET and lower dose. However, when PTIO, a nitric oxide (NO)-specific scavenger, was present in the co-culture medium, the cell growth capacity of the unirradiated recipients was reduced to control level, indicating that NO is involved in the bystander response. As an oxidization product of NO, nitrite was detected in the co-culture medium and its concentration depended on the LET and dose of irradiation. Using a NO-generator sper/NO, it was verified that NO at low concentrations indeed enhanced cell proliferation. Accordingly, NO plays an important role in medium-mediated bystander effects.