Social environment modulates anxiogenic effects of caffeine in zebrafish
Social environment modulates anxiogenic effects of caffeine in zebrafish
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DOI：
10.1097/fbp.0000000000000415
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发表时间：
2019-02-01
影响因子：
1.6
通讯作者：
Porfiri, Maurizio
中科院分区：
文献类型：
--
作者：
Neri, Daniele;Ruberto, Tommaso;Porfiri, Maurizio
斑马鱼最近已经成为一些临床前研究的首选物种，这些研究旨在研究精神活性化合物是否以及如何调节个人和社会行为。在这里，我们试图更好地了解咖啡因对斑马鱼游泳活动和集体反应的影响。咖啡因是一种众所周知的导致焦虑的化合物。为了研究社会环境如何影响个体对急性咖啡因治疗的反应，我们测量了接受咖啡因治疗的受试者在单独游泳或在一组未治疗的同种动物在场的情况下的行为。实验范式使用了最近开发的自动跟踪系统来提取斑马鱼的个体轨迹，并随着时间的推移保存它们的身份。我们的结果表明，咖啡因减少了单独测试的鱼的游泳活动，这种影响可以通过未处理的同种物质的存在而减轻。集体反应也受到低剂量咖啡因暴露的影响，在这种情况下，接受咖啡因治疗的受试者可能会作为小组领导者，采取预期的转弯动作，随后小组的其他成员也会跟随。虽然咖啡因的焦虑效应可以解释游泳活动的减少，但观察到的集体反应的变化可能与咖啡因的焦虑和精神刺激特性之间的相互作用有关。版权所有(C)2018 Wolters Kluwer Health，Inc.保留所有权利。
Zebrafish has recently become a species of choice in a number of preclinical studies to examine whether and how psychoactive compounds modulate individual and social behaviors. Here, we sought to contribute an improved understanding of the effects of caffeine, a well-known anxiogenic compound, on the swimming activity and the collective response of zebrafish. To investigate how the social environment influences individual response to acute caffeine treatment, we measured the behavior of a caffeine-treated subject swimming in isolation or in the presence of a group of untreated conspecifics. The experimental paradigm used a recently developed automatic tracking system to extract individual trajectories of zebrafish and preserve their identities over time. Our results indicate that caffeine reduces the swimming activity of fish tested in isolation, and that this effect is mitigated by the presence of untreated conspecifics. The collective response is also influenced by caffeine exposure at low doses, whereby caffeine-treated subjects may act as group leaders by taking anticipatory turning manoeuvres that are followed by the rest of the group. While anxiogenic effects of caffeine could explain the reduction in the swimming activity, the observed variation in the collective response is likely related to the interplay between anxiogenic and psychostimulant properties of caffeine. Copyright (c) 2018 Wolters Kluwer Health, Inc. All rights reserved.