Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea
Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea
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DOI：
10.1016/j.dsr.2011.07.003
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发表时间：
2011-10-01
影响因子：
2.4
通讯作者：
Stoeck, T.
中科院分区：
文献类型：
--
作者：
Edgcomb, V. P.;Orsi, W.;Stoeck, T.
接近饱和的盐浓度和相应的高密度、高静水压力、缺乏光线、缺氧和尖锐的化学跃层相结合，使地中海东部的深部高盐度缺氧盆地成为地球上最极端的栖息地之一。使用动质体特异性引物，我们在一些迄今已知的最恶劣的深海环境中检测到动质体鞭毛虫，包括一些其小亚单位核糖体RNA基因序列与培养代表没有密切关系的环境。动植体，包括可能是新的代表，似乎是东地中海盐跃层环境的专家，并构成了几个盆地的卤水和盐跃层中原生生物群落的很大一部分。荧光原位杂交数据显示，在发现盆地盐跃层中，一种新的与半身类有关的动质体动质体序列分支占整个原生生物群落的10%。不同的动质体群在我们采样的不同盆地和生境中表现不均匀，我们讨论了这一点是环境选择的结果。(C)2011爱思唯尔有限公司。保留所有权利。
The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Fluorescent in situ hybridization data indicate a novel 'unidentified' sequence clade of kinetoplastids related to bodonids represents as much as 10% of the total protist community in the Discovery Basin halocline. Different kinetoplastid groups are unevenly represented in the different basins and habitats we sampled, which we discuss as a result of environmental selection. (C) 2011 Elsevier Ltd. All rights reserved.