Cellular localization of an Epstein‐Barr virus (EBV)‐associated complement‐fixing antigen in producer and non‐producer lymphoblastoid cell lines
Cellular localization of an Epstein‐Barr virus (EBV)‐associated complement‐fixing antigen in producer and non‐producer lymphoblastoid cell lines
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EB病毒（EBV）相关补体固定抗原在生产者和非生产者淋巴母细胞系中的细胞定位
DOI：
10.1002/ijc.2910110302
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发表时间：
1973
影响因子：
6.4
通讯作者：
G. Klein
中科院分区：
文献类型：
--
作者：
B. Reedman;G. Klein
抗补体免疫荧光（ACIF）用于研究人淋巴母细胞样细胞系的补体结合抗原。这些细胞系携带爱泼斯坦-巴尔病毒（EBV）基因组，尽管只有生产者培养物合成EBV特异性抗原（病毒衣壳抗原，VCA和早期抗原，EA），可通过直接和间接免疫荧光检测，通常不到5%的细胞。ACIF试验显示抗原定位于淋巴母细胞样细胞的细胞核中。与EA和VCA相反，该抗原存在于生产者和非生产者培养物的90%以上的细胞中。通过比较52份血清在ACIF试验中的反应，表明该抗原对EBV具有特异性。给予核反应的血清含有VCA、EA或抗原的抗体，可通过对细胞提取物的补体结合试验检测到，但不含EBV抗体的血清不能给予反应。在VCA、EA或补体结合试验中，6份低滴度血清出现弱、不明确或不一致的反应。通过EBV转化人和灵长类动物淋巴细胞获得的细胞系给出了核反应。与EBV无关的对照细胞无反应性。这些包括由植物血凝素转化的胎儿淋巴细胞，来自乳腺癌、神经胶质瘤、正常神经胶质、恶性胸膜炎和骨髓瘤的细胞系，以及两种携带松鼠猴疱疹病毒（HVS）的绒猴淋巴系。在初步实验中，ACIF测试被用作在细胞水平上追踪EBV基因组的工具。来自两个伯基特淋巴瘤活检组织的细胞，一个在活检后检测，一个在裸鼠中传代后检测，含有EBV特异性抗原。来自小鼠体细胞和人淋巴母细胞系的杂交细胞的三个克隆也含有这种抗原，但反应性细胞的数量因克隆而异。第四个克隆无反应。
Anti‐complement immunofluorescence (ACIF) was used to study the complementfixing antigens of human lymphoblastoid cell lines. These cell lines carry the Epstein‐Barr virus (EBV) genome although only producer cultures synthetize EBV‐specific antigens (virus capsid antigen, VCA and early antigen, EA) detectable by direct and indirect immunofluorescence, usually in less than 5% of the cells. The ACIF test revealed an antigen localized in the nucleus of the lymphoblastoid cells. In contrast to EA and VCA, this antigen was present in over 90% of the cells of both producer and non‐producer cultures. The antigen was shown to be specific for EBV by comparing the reactions of 52 sera in the ACIF test. Sera giving the nuclear reaction contained antibodies to VCA, EA or antigens detectable by complement fixation tests on cell extracts, but sera without EBV antibodies failed to give the reaction. Weak, equivocal or discordant reactions occurred with six sera with low titres in VCA, EA or complement fixation tests. Cell lines derived by transformation of human and primate lymphocytes by EBV gave the nuclear reaction. Control cells with no known association with EBV were non‐reactive. These included foetal lymphocytes transformed by phytohaemagglutinin, cell lines derived from breast cancer, glioma, normal glia, pleuritis maligna and myeloma, and two marmoset lymphoid lines carrying Herpesvirus saimiri (HVS). In preliminary experiments, the ACIF test was used as a tool to trace the EBV genome at the cellular level. Cells from two Burkitt lymphoma biopsies, one tested after biopsy and one after passaging in nude mice, contained an EBV‐specific antigen. Three clones of cells derived from hybrids of mouse somatic cells and a human lymphoblastoid cell line also contained such an antigen, but the number of reactive cells varied from clone to clone. A fourth clone was non‐reactive.