Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line (PMDC05)
Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line (PMDC05)
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DOI：
10.1016/j.leukres.2010.03.002
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发表时间：
2010-11-01
影响因子：
2.7
通讯作者：
Takahashi, Masuhiro
中科院分区：
文献类型：
--
作者：
Watanabe, Norihiro;Narita, Miwako;Takahashi, Masuhiro
我们研究了在我们实验室从一位pDC白血病患者建立的pDC系（PMDC05）中pDC向mdc的转化。根据BDCA1和CD123的表达情况将PMDC05细胞分为两组。BDCA1（-）和CD123（+）细胞的形态、表面表型、mRNA表达和功能均显示为pdc样细胞。此外，BDCA1(-)CD123（+）细胞被证明具有增殖能力，并显示出转化为具有mdc样特性的BDCA1(+)CD123（-）细胞的能力。我们的数据证明了人类DC谱系中pDCs向mdc转化的可能性。(C) 2010 Elsevier Ltd.版权所有。
We investigated the transformation from pDCs to mDCs in a pDC line (PMDC05) which was established from a patient with pDC leukemia in our laboratory. PMDC05 cells were separated into two fractions according to the expression of BDCA1 and CD123. BDCA1(-)CD123(+) cells were found to be pDC-like cells by their morphology, surface phenotypes, mRNA expression and the function. In addition, BDCA1(-)CD123(+) cells were demonstrated to have a proliferating capacity and revealed the ability to transform to BDCA1(+)CD123(-) cells which showed mDC-like properties. Our data demonstrated the possibility of transformation from pDCs to mDCs in human DC lineage. (C) 2010 Elsevier Ltd. All rights reserved.