Differential expression of prolactin receptor (PRLR) in normal and tumorous adrenal tissues: separation of cellular endocrine compartments by laser capture microdissection (LCM).
Differential expression of prolactin receptor (PRLR) in normal and tumorous adrenal tissues: separation of cellular endocrine compartments by laser capture microdissection (LCM).
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催乳素受体（PRLR）在正常和肿瘤肾上腺组织中的差异表达：通过激光捕获显微切割（LCM）分离细胞内分泌室。
DOI：
10.3109/07435809809032697
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发表时间：
1998
影响因子：
2.1
通讯作者：
S. Bornstein
中科院分区：
文献类型：
--
作者：
A. Glasow;A. Haidan;J. Gillespie;P. Kelly;G. Chrousos;S. Bornstein
PRL 刺激肾上腺类固醇生成。在本研究中，我们比较了正常和肿瘤肾上腺组织中的 PRLR 表达，并研究了 PRL 在肾上腺细胞中的潜在增殖作用。在正常肾上腺皮质、良性和恶性肾上腺肿瘤以及人肾上腺皮质癌细胞系 NCI-H295 中检测到长型和中间型 PRLR 的 mRNA 表达。 LCM 获得的细胞的分子分析清楚地表明 PRLR mRNA 在肾上腺皮质中表达，但在髓质中不表达。免疫染色显示正常肾上腺皮质的所有三个区域均存在 PRLR 蛋白。此外，肾上腺癌和腺瘤的 PRLR 染色呈阳性，而在嗜铬细胞瘤和正常肾上腺髓质中，没有观察到特异性染色。通过WST-1试验，我们可以发现PRL（10（-7）M）降低了原代细胞培养物中肾上腺细胞的增殖和活力，表明PRL不是肾上腺皮质细胞的有丝分裂因子。
PRL stimulates adrenal steroidogenesis. In this study, we compared the PRLR expression in normal and tumorous adrenal tissues and investigated a potential proliferative effect of PRL in adrenal cells. mRNA expression of long and intermediate forms of PRLR was detected in both normal adrenal cortex as well as benign and malignant adrenal tumors and in the human adrenocortical carcinoma cell line NCI-H295. Molecular analysis of cells procured by LCM clearly demonstrated that PRLR mRNA is expressed in the adrenal cortex but not in the medulla. Immunostaining revealed PRLR protein in all three zones of the normal adrenal cortex. Furthermore, adrenal carcinomas and adenomas stained positive for the PRLR, while in phaeochromocytomas as in the normal adrenal medulla, no specific staining was observed. By WST-1 test, we could show that PRL (10(-7) M) decreased proliferation and viability of adrenal cells in primary cell culture suggesting that PRL is not a mitogenic factor of adrenocortical cells.
催乳素及其受体在人乳腺癌中的表达。
影响因子：
4.8
作者：
Reynolds,C;Montone,KT;Powell,CM;Tomaszewski,JE;Clevenger,CV
通讯作者：
Clevenger,CV