A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain
A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain
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DOI：
10.1016/s0016-5085(97)70155-0
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发表时间：
1997-04-01
期刊：
影响因子：
29.4
通讯作者：
Dixon, MF
中科院分区：
文献类型：
--
作者：
Lee, A;ORourke, J;Dixon, MF
背景与目的：目前可用的幽门螺杆菌模型显示出不同的定植，在某些情况下，定植不良。需要一种具有高定殖能力的菌株作为动物研究的标准。方法：通过对一系列新鲜临床分离株的筛选和小鼠的长期适应，分离出一株具有很好定殖能力的幽门螺杆菌。结果：该菌株命名为幽门螺杆菌悉尼菌株（菌株SS1）， cagA和vacA阳性。高水平的定植(10（6)-10(7)个单位/g组织）在C57BL/6小鼠中一致实现。定植水平取决于使用BALB/c、DBA/2和C3H/He的小鼠品系，它们都被定植，但数量较少。在所有品系的小鼠中，细菌在胃窦和身体之间的交界处清晰可见。体外传代20次后表型稳定，仍有定殖能力。这种细菌牢固地附着在胃上皮上。8个月后，C57BL/6和BALB/c小鼠慢性活动性胃炎缓慢发展，进展为严重萎缩。结论：悉尼幽门螺杆菌菌株适用于所有人，并将为疫苗开发、化合物筛选和发病机制研究提供标准化的小鼠模型。
Background & Aims: Currently available Helicobacter pylori models show variable and, in some instances, poor colonization. There is a need for a strain with high colonizing ability to act as a standard for animal studies. Methods: After screening a range of fresh clinical isolates and long-term adaptation in mice, a strain of H. pylori has been isolated with a very good colonizing ability. Results: This strain, named the Sydney strain of H. pylori (strain SS1), is cagA and vacA positive. High levels of colonization (10(6)-10(7) colony-forming units/g tissue) were achieved consistently in C57BL/6 mice. Colonization levels varied depending on the mouse strain used with BALB/c, DBA/2, and C3H/He, all being colonized but in lower numbers. In all strains of mice, bacteria were clearly visible at the junctional zone between the antrum and the body. The phenotype was stable with colonizing ability remaining after 20 subcultures in vitro. The bacterium attached firmly to gastric epithelium. During 8 months, a chronic active gastritis slowly developed, progressing to severe atrophy in both C57BL/6 and BALB/c mice. Conclusions: The Sydney strain of H. pylori is available to all and will provide a standardized mouse model for vaccine development, compound screening, and studies in pathogenesis.