Variation in expression of the HECT E3 ligase UPL3 modulates LEC2 levels, gene expression, seed size and crop yields in Brassica napus
Variation in expression of the HECT E3 ligase UPL3 modulates LEC2 levels, gene expression, seed size and crop yields in Brassica napus
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DOI：
10.1101/334581
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发表时间：
2018-05
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通讯作者：
Charlotte Miller;R. Wells;N. McKenzie;M. Trick;Joshua Ball;Abdelhak Fatihi;Bertrand Debreuq;T. Chardot;L. Lepiniec;M. Bevan
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作者：
Charlotte Miller;R. Wells;N. McKenzie;M. Trick;Joshua Ball;Abdelhak Fatihi;Bertrand Debreuq;T. Chardot;L. Lepiniec;M. Bevan
识别提高作物产量的遗传变异是植物育种的主要目标。我们使用甘蓝型油菜（油菜/双低油菜）种质的关联分析来鉴定影响种子大小和最终产量的 HECT E3 连接酶基因 BnaUPL3.C03 表达的变异。我们建立了一种机制，其中 UPL3 介导 LEC2（种子成熟的主要调节因子）的蛋白酶体降解。 UPL3 表达减少会增加 LEC2 蛋白水平，并延长脂质生物合成基因的表达和种子成熟。 BnaUPL3.C03 表达的自然变异尚未在当前的甘蓝型油菜育种系中得到利用，因此可以用作最大化这一重要油料作物未来产量的新方法。
Identifying genetic variation that increases crop yields is a primary objective in plant breeding. We have used association analyses of Brassica napus (oilseed rape/canola) accessions to identify variation in the expression of a HECT E3 ligase gene, BnaUPL3.C03, that influences seed size and final yield. We establish a mechanism in which UPL3 mediates the proteasomal degradation of LEC2, a master regulator of seed maturation. Reduced UPL3 expression increases LEC2 protein levels and prolongs expression of lipid biosynthetic genes and seed maturation. Natural variation in BnaUPL3.C03 expression has not yet been exploited in current Brassica napus breeding lines and can therefore be used as a new approach to maximize future yields in this important oil crop.