Stem Cells Marked by the R-Spondin Receptor LGR5
Stem Cells Marked by the R-Spondin Receptor LGR5
复制标题
DOI：
10.1053/j.gastro.2014.05.007
复制
发表时间：
2014-08-01
期刊：
影响因子：
29.4
通讯作者：
Clevers, Hans
中科院分区：
文献类型：
--
作者：
Koo, Bon-Kyoung;Clevers, Hans
自从发现 LGR5 作为肠道干细胞的标记以来，该领域得到了爆炸性的发展，并带来了许多新的研究途径。肠隐窝干细胞生态位的内部运作现已被充分了解。对干细胞富集基因的研究揭示了 Wnt 信号通路的一些以前未知的方面，Wnt 信号通路是隐窝动力学的主要驱动因素。 LGR5(+) 干细胞现在可以作为上皮类器官或“迷你肠道”在体外长期培养。这项技术为使用培养的成体干细胞进行药物开发、疾病建模、基因治疗和再生医学开辟了新的可能性。这篇综述描述了隐窝基底柱状细胞作为 LGR5(+) 成体干细胞的重新发现，并总结了该领域的后续进展、前景、未解决的问题和挑战。
Since the discovery of LGR5 as a marker of intestinal stem cells, the field has developed explosively and led to many new avenues of research. The inner workings of the intestinal crypt stem cell niche are now well understood. The study of stem cell-enriched genes has uncovered some previously unknown aspects of the Wnt signaling pathway, the major driver of crypt dynamics. LGR5(+) stem cells can now be cultured over long periods in vitro as epithelial organoids or "mini-guts." This technology opens new possibilities of using cultured adult stem cells for drug development, disease modeling, gene therapy, and regenerative medicine. This review describes the rediscovery of crypt base columnar cells as LGR5(+) adult stem cells and summarizes subsequent progress, promises, unresolved issues, and challenges of the field.