Array biosensor for simultaneous identification of bacterial, viral, and protein analytes
Array biosensor for simultaneous identification of bacterial, viral, and protein analytes
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DOI：
10.1021/ac981425v
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发表时间：
1999-09-01
影响因子：
7.4
通讯作者：
Ligler, FS
中科院分区：
文献类型：
--
作者：
Rowe, CA;Tender, LM;Ligler, FS
阵列生物传感器被制造成同时分析多个样品中的多个分析物。传感器采用标准夹心免疫测定形式：抗原特异性“捕获”抗体固定在平面波导表面上的图案化阵列中，随后使用荧光示踪抗体检测结合的分析物。本研究描述了对126份盲样进行的三种不同类型分析物的分析。为了解决在多分析物测定中使用示踪抗体混合物引起的潜在并发症，三种单分析物测定与多分析物测定平行运行。分析物的混合物也进行了测定，以证明传感器的能力，以检测一次超过一个单一的物种。该阵列传感器能够检测病毒，细菌和蛋白质分析物，使用一个简单的14分钟的测定与接近标准ELISA方法的灵敏度水平。球形芽孢杆菌、MS 2噬菌体和葡萄球菌肠毒素B（SE B）的检测限分别为10（5）cfu/mL、10（7）pfu/mL和10 ng/mL。阵列生物传感器还同时分析多个样品，并检测多分析物格式中不同类型分析物的混合物。
The array biosensor was fabricated to analyze multiple samples simultaneously for multiple analytes. The sensor utilized a standard sandwich immunoassay format: Antigen-specific "capture" antibodies were immobilized in a patterned array on the surface of a planar waveguide and bound analyte was subsequently detected using fluorescent tracer antibodies. This study describes the analysis of 126 blind samples for the presence of three distinct classes of analytes. To address potential complications arising from using a mixture of tracer antibodies in the multianalyte assay, three single-analyte assays were run in parallel with a multianalyte assay. Mixtures of analytes were also assayed to demonstrate the sensor's ability to detect more than a single species at a time. The array sensor was capable of detecting viral, bacterial, and protein analytes using a facile 14-min assay with sensitivity levels approaching those of standard ELISA methods. Limits of detection for Bacillus globigii, MS2 bacteriophage, and staphylococcal enterotoxin B (SEB) were 10(5) cfu/mL, 10(7) pfu/mL, and 10 ng/mL, respectively, The array biosensor also analyzed multiple samples simultaneously and detected mixtures of the different types of analytes in the multianalyte format.