Barbed micro-spikes for micro-scale biopsy
Barbed micro-spikes for micro-scale biopsy
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DOI：
10.1088/0960-1317/15/6/020
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发表时间：
2005-06-01
影响因子：
2.3
通讯作者：
Cho, D
中科院分区：
文献类型：
--
作者：
Byun, S;Lim, JM;Cho, D
研制了一种用于显微活检的单晶硅平面微刺，首次评价了该微刺作为显微活检工具的可行性。制造过程利用深硅蚀刻来限定微钉轮廓，从而产生各种形状的突出倒钩。所制造的微钉的杆长3 mm，厚100 gm，宽250 gm。从微穿刺器柄突出的倒钩通过从靶组织撕下并保留样本来促进活检程序。带倒刺的微刺成功地从麻醉猪的小肠中提取了组织样本，而不带倒刺的微刺未能获得活检样本。聚对二甲苯涂层可用于改善微刺的生物相容性，而不会劣化微刺的活检功能。此外，为了表明具有微刺的活检可以应用于组织分析，使用免疫荧光染色检查通过微刺获得的样品。通过免疫荧光染色，可以清楚地观察到由来自transwell的微刺提取的细胞的细胞核和F-肌动蛋白。
Single-crystal silicon planar micro-spikes with protruding barbs are developed for micro-scale biopsy and the feasibility of using the micro-spike as a micro-scale biopsy tool is evaluated for the first time. The fabrication process utilizes a deep silicon etch to define the micro-spike outline, resulting in protruding barbs of various shapes. Shanks of the fabricated micro-spikes are 3 mm long, 100 gm thick and 250 gm wide. Barbs protruding from micro-spike shanks facilitate the biopsy procedure by tearing off and retaining samples from target tissues. Micro-spikes with barbs successfully extracted tissue samples from the small intestines of the anesthetized pig, whereas micro-spikes without barbs failed to obtain a biopsy sample. Parylene coating can be applied to improve the biocompatibility of the micro-spike without deteriorating the biopsy function of the micro-spike. In addition, to show that the biopsy with the micro-spike can be applied to tissue analysis, samples obtained by micro-spikes were examined using immunofluorescent staining. Nuclei and F-actin of cells which are extracted by the micro-spike from a transwell were clearly visualized by immunofluorescent staining.