Mini-mouse: disruption of the pygmy locus in a transgenic insertional mutant.
Mini-mouse: disruption of the pygmy locus in a transgenic insertional mutant.
复制标题
迷你小鼠：转基因插入突变体中侏儒位点的破坏。
DOI：
10.1126/science.2305264
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Chada,K
中科院分区：
文献类型：
--
作者：
Xiang,X;Benson,KF;Chada,K
一个创始人转基因小鼠窝藏两个不同的整合模式的转基因在同一个基因座，每一个都引起了一个类似的常染色体隐性突变。突变表型的小鼠身材矮小，但生长激素水平正常。克隆了被破坏的基因座，遗传和分子分析表明，插入突变体与自发突变体pygmy是等位的。小鼠应该是一个有用的模型，用于抗生长激素的人类侏儒综合征，并可能导致更好地了解参与生长和发育的途径。
A founder transgenic mouse harbored two different integration patterns of a transgene at the same locus, each of which gave rise to a similar autosomal recessive mutation. Mice of the mutant phenotype were of small stature but had normal levels of growth hormone. The disrupted locus was cloned, and a genetic and molecular analysis showed that the insertional mutants were allelic to a spontaneous mutant, pygmy. The mice should be a useful model for the growth hormone-resistant human dwarf syndromes and could lead to a greater understanding of the pathways involved in growth and development.