A molecular atlas of cell types and zonation in the brain vasculature
A molecular atlas of cell types and zonation in the brain vasculature
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DOI：
10.1038/nature25739
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发表时间：
2018-02-22
期刊：
影响因子：
64.8
通讯作者：
Betsholtz, Christer
中科院分区：
文献类型：
--
作者：
Vanlandewijck, Michael;He, Liqun;Betsholtz, Christer
脑血管疾病是发达国家第三大常见死因，但我们对构成脑血管系统的细胞的了解有限。在这里，使用血管单细胞转录组学，我们提供了成年小鼠大脑中主要类型的血管和血管相关细胞的分子定义。我们揭示了转录的基础上逐渐表型变化（带状）沿着动静脉轴，并揭示意想不到的细胞类型的差异：一个无缝的连续内皮细胞与间断连续壁细胞。我们还提供了深入了解周细胞器官型，并定义了一个人口的血管周围成纤维细胞样细胞，除了毛细血管的所有类型的血管。我们的工作说明了单细胞转录组学解码组织的更高组织原则的能力，并可能提供哺乳动物脉管系统的分子百科全书的第一章。
Cerebrovascular disease is the third most common cause of death in developed countries, but our understanding of the cells that compose the cerebral vasculature is limited. Here, using vascular single-cell transcriptomics, we provide molecular definitions for the principal types of blood vascular and vessel-associated cells in the adult mouse brain. We uncover the transcriptional basis of the gradual phenotypic change (zonation) along the arteriovenous axis and reveal unexpected cell type differences: a seamless continuum for endothelial cells versus a punctuated continuum for mural cells. We also provide insight into pericyte organotypicity and define a population of perivascular fibroblast-like cells that are present on all vessel types except capillaries. Our work illustrates the power of single-cell transcriptomics to decode the higher organizational principles of a tissue and may provide the initial chapter in a molecular encyclopaedia of the mammalian vasculature.