Diversity of Kale (Brassica oleracea var. sabellica): Glucosinolate Content and Phylogenetic Relationships
Diversity of Kale (Brassica oleracea var. sabellica): Glucosinolate Content and Phylogenetic Relationships
复制标题
DOI：
10.1021/acs.jafc.6b01000
复制
发表时间：
2016-04-27
影响因子：
6.1
通讯作者：
Albach, Dirk
中科院分区：
文献类型：
--
作者：
Hahn, Christoph;Mueller, Anja;Albach, Dirk
最近，羽衣甘蓝因其有益人体健康的营养成分而受到欢迎。它是硫代葡萄糖苷等植物化学物质的重要来源，可引发相关的癌症预防活动。然而，硫代葡萄糖苷的营养价值因品种而异。本文采用HPLC-DAD-ESI-MSn对25个羽衣甘蓝品种和11个非羽衣甘蓝品种的5种硫代葡萄糖苷含量进行了系统测定，并与KASP基因分型分析的snp分析结果进行了比较。结果表明，不同产地品种的硫代葡萄糖苷含量差异显著。植物化学数据与系统发育关系的比较表明，共同名称羽衣甘蓝至少涉及三个不同的类群。德国、美国和意大利羽衣甘蓝在形态和植物化学上有所不同。地方品种没有显著的硫代葡萄糖苷水平。我们的研究结果证明了羽衣甘蓝的多样性以及为未来育种目的保留广泛基因库的重要性。
Recently, kale has become popular due to nutritive components beneficial for human health. It is an important source of phytochemicals such as glucosinolates that trigger associated cancer-preventive activity. However, nutritional value varies among glucosinolates and among cultivars. Here, we start a systematic determination of the content of five glucosinolates in 25 kale varieties and 11 non-kale Brassica oleracea cultivars by HPLC-DAD-ESI-MSn and compare the profiles with results from the analysis of SNPs derived from a KASP genotyping assay. Our results demonstrate that the glucosinolate levels differ markedly among varieties of different origin. Comparison of the phytochemical data with phylogenetic relationships revealed that the common name kale refers to at least three different groups. German, American, and Italian kales differ morphologically and phytochemically. Landraces do not show outstanding glucosinolate levels. Our results demonstrate the diversity of kale and the importance of preserving a broad genepool for future breeding purposes.