Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia
Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia
复制标题
DOI：
10.1182/blood.v97.7.2115
复制
发表时间：
2001-04-01
期刊：
影响因子：
20.3
通讯作者：
Campana, D
中科院分区：
文献类型：
--
作者：
Chen, JS;Coustan-Smith, E;Campana, D
为了鉴定b系急性淋巴细胞白血病（ALL）微小残留病（MRD）的新标志物，将4例新诊断的ALL患者的白血病细胞与2例健康供者的正常CD19(+)CD10(+) b细胞祖细胞的基因表达进行比较，通过cDNA阵列分析，研究的4132个基因中有334个在白血病细胞中的表达比正常样本高1.5- 5.8倍；其中238个基因在白血病细胞系RS4中也过表达；11、从274个过表达基因中选择9个在至少2个白血病样本中表达的基因，流式细胞术检测编码蛋白的表达，结果显示，尽管阵列中有很强的杂交信号，但2个蛋白（caldesmon和髓样核分化抗原）在白血病细胞中仅弱表达。相比之下，7种蛋白（CD58、肌酸激酶B、ninjurin1、Ref1、calpastatin、HDJ-2和annexin VI）在B系ALL细胞中的表达水平高于正常CD19(+)CD10(+) B细胞祖细胞(P
To identify new markers of minimal residual disease (MRD) in B-lineage acute lymphoblastic leukemia (ALL), gene expression of leukemic cells obtained from 4 patients with newly diagnosed ALL was compared with that of normal CD19(+)CD10(+) B-cell progenitors obtained from 2 healthy donors, By cDNA array analysis, 334 of 4132 genes studied were expressed 1.5- to 5.8-fold higher in leukemic cells relative to both normal samples; 238 of these genes were also overexpressed in the leukemic cell line RS4;11, Nine genes were selected among the 274 overexpressed in at least 2 leukemic samples, and expression of the encoded proteins was measured by flow cytometry, Two proteins (caldesmon and myeloid nuclear differentiation antigen) were only weakly expressed in leukemic cells despite strong hybridization signals in the array. By contrast, 7 proteins (CD58, creatine kinase B, ninjurin1, Ref1, calpastatin, HDJ-2, and annexin VI) were expressed in B-lineage ALL cells at higher levels than in normal CD19(+)CD10(+) B-cell progenitors (P