Protein Adsorption at the Solid-Solution Interface in Relation to Blood-Material Interactions
Protein Adsorption at the Solid-Solution Interface in Relation to Blood-Material Interactions
复制标题
与血液-物质相互作用相关的固溶体界面的蛋白质吸附
DOI：
10.1021/bk-1987-0343.ch030
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
J. Brash
中科院分区：
文献类型：
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作者：
J. Brash
综述了作者利用放射性标记、流体力学厚度测量和洗脱/鉴定方法研究血浆蛋白吸附的工作。这项工作的动机是需要了解蛋白质吸附在血液-异物表面接触后血栓形成中的作用。对单一蛋白质体系的研究表明，吸附发生在单层中，这种吸附是固有的，但只能缓慢地可逆，而且吸附后蛋白质结构会发生一些变化。与蛋白质混合物的研究表明，相对于白蛋白和免疫球蛋白，纤维蛋白原具有很强的优先吸附作用。从血浆中，相对缺乏对丰富蛋白质的吸附。纤维蛋白原从血浆中的吸附是暂时的，它被接触相凝血蛋白所取代。从血浆中吸附的部分纤维蛋白原被表面产生的纤溶酶降解，这意味着表面具有溶栓和血栓形成的特性。
The author's work on the adsorption of plasma proteins using radiolabeling, hydrodynamic thickness measurements and elution/identification methods is reviewed. This work was motiviated by the need to understand the role of protein adsorption in thrombus formation following blood-foreign surface contact. Studies of single protein systems showed that adsorption occurs in monolayers, that adsorption is inherently, but only slowly reversible and that some alterations of protein structure can occur upon adsorption. Work with protein mixtures showed the strong preferential adsorption of fibrinogen relative to albumin and IgG. From plasma there is a relative lack of adsorption of the abundant proteins. Adsorption of fibrinogen from plasma was shown to be transient, it is displaced by contact phase clotting proteins. Part of the fibrinogen adsorbed from plasma was found to be degraded by surface generated plasmin implying that surfaces have thrombolytic as well as thrombogenic properties.