Immunolocalization of DCAMKL-1, a putative intestinal stem cell marker, in normal colonic tissue
Immunolocalization of DCAMKL-1, a putative intestinal stem cell marker, in normal colonic tissue
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DOI：
10.1016/j.prp.2012.05.015
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Bellows, Charles F.
中科院分区：
文献类型：
--
作者：
Gagliardi, Giuseppe;Moroz, Krzysztof;Bellows, Charles F.
双皮质素和CaM激酶样1 （DCAMKL-1）是一种微管相关蛋白激酶，最近被提出作为胃肠道干细胞标志物。本研究的目的是通过免疫组织化学方法表征DCAMKL-1在正常人结肠中的表达。采用DCAMKL-1免疫染色对14例组织学正常的结直肠活检组织进行染色。超过60%的结肠隐窝存在免疫反应性，表现为细胞质染色强，分布抗腔，使人联想到神经内分泌细胞的细胞质取向。DCAMKL-1阳性细胞在隐窝基部的前4个细胞中最多（48.5%）。70%的DCAMKL-1阳性细胞位于隐窝的下三分之一，26%位于中三分之一，4%位于上三分之一。因此，在正常结肠黏膜中，DCAMKL-1的表达并不局限于干细胞区室。当我们将DCAMKL-1免疫染色与富含亮氨酸的含有重复序列的g蛋白偶联受体5 (Lgr5)（一种肠道干细胞标记物）和铬粒蛋白- a（一种肠内分泌细胞标记物）的免疫染色进行比较时，我们发现DCAMKL-1阳性细胞仅在隐窝基部与Lgr5共染色，但在整个隐窝与CgA共染色。我们的研究结果表明，DCAMKL-1标志着结直肠干细胞的一个亚群，以及肠内分泌细胞的一个亚群。Elsevier GmbH出版。
Doublecortin and CaM kinase-like-1 (DCAMKL-1) is a microtubule-associated protein kinase which has been recently proposed as a gastrointestinal stem cell marker. The aim of our study was to characterize DCAMKL-1 expression in normal human colon by immunohistochemistry. DCAMKL-1 immunostaining was performed on histologically normal colorectal biopsies from 14 patients. Immunoreactivity was found in over 60% of colonic crypts and was represented by strong cytoplasmic staining, anti-luminal in distribution, and was reminiscent of cytoplasmic orientation of neuroendocrine cells. The highest number (48.5%) of DCAMKL-1 positive cells was found in the first 4 cells from the crypt base. Seventy percent of DCAMKL-1 positive cells were located in the lower third of the crypt, 26% in the middle third and 4% in the upper third. Therefore, in normal colonic mucosa, expression of DCAMKL-1 is not confined to the stem cell compartment. When we compared DCAMKL-1 immunostaining with that of the leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5), an intestinal stem cell marker, and chromogranin-A (CgA), an enteroendocrine cell marker, we found that DCAMKL-1 positive cells co-stained with Lgr5 only at the crypt base, but co-stained with CgA throughout the crypt. Our findings suggest that DCAMKL-1 marks a subset of colorectal stem cells, as well as a subset of enteroendocrine cells. Published by Elsevier GmbH.