Visibility Enhancement by Integrating Refocusing and Direct-Global Separation with Contact Imaging
Visibility Enhancement by Integrating Refocusing and Direct-Global Separation with Contact Imaging
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DOI：
10.1007/s11263-019-01173-5
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发表时间：
2019-03
影响因子：
19.5
通讯作者：
Fusataka Kuniyoshi;Takuya Funatomi;Hiroyuki Kubo;Yoshihide Sawada;Y. Kato;Y. Mukaigawa
中科院分区：
文献类型：
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作者：
Fusataka Kuniyoshi;Takuya Funatomi;Hiroyuki Kubo;Yoshihide Sawada;Y. Kato;Y. Mukaigawa
接触成像是一种紧凑型无透镜显微镜技术，可以观察直接放置在电荷耦合器件传感器上的活细胞。然而，捕获的图像通常表现出较低的可见度。这项工作的目的是将计算重聚焦和高频照明集成到接触成像中，以增强捕获图像的可见度。重新聚焦有助于从移动点光源捕获的光场合成浅景深图像。可以通过高频照明来增强可见度。两种技术的简单集成需要进行多次观察。因此，我们提出通过重新制定接触成像中应用的计算重聚焦来有效整合减少的观察结果。此外，我们开发了一个原型系统，证明了所提出方法的有效性。
Contact imaging is a compact lensless microscopy technique that allows observation of living cells placed directly on a charge-coupled device sensor. However, captured images exhibit low visibility in general. This work is aimed at integrating computational refocusing and high-frequency illumination into contact imaging to enhance the visibility of the captured images. Refocusing contributes to the synthesis of shallow depth-of-field images from the light field captured by moving a point light source. The visibility may be enhanced via high-frequency illumination. Naïve integration of both techniques requires several observations. Therefore, we propose an efficient integration from reduced observations by reformulating the computational refocusing applied in contact imaging. Furthermore, we developed a prototype system that demonstrates the effectiveness of the proposed method.