Intracellular recordings from rat thalamic VL neurons: a study combined with intracellular staining
Intracellular recordings from rat thalamic VL neurons: a study combined with intracellular staining
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大鼠丘脑 VL 神经元的细胞内记录：结合细胞内染色的研究
DOI：
10.1007/bf00231841
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发表时间：
2004
影响因子：
2
通讯作者：
Hiroshi Oka
中科院分区：
文献类型：
--
作者：
Tetsuro Yamamoto;Yuka Kishimoto;Hideki Yoshikawa;Hiroshi Oka
在乌拉坦麻醉下，对接受小脑输入的大鼠丘脑神经元进行了细胞内记录。用装有4%生物胞素溶液（溶于0.5M醋酸钾）的微量移液管在细胞内记录共64个神经元，并通过细胞内电流注入分析小脑诱导的EPSP（CN-EPSP）的膜电阻和放电特性。CN-EPSP的平均潜伏期为1.9 ± 0.8ms，10个神经元的平均输入电阻为17.6 ± 5.0M Ω。35个染色的神经元中有32个进行了形态学分析;其中28个位于VL，26个接受CN-EPSP。胞体圆形或多边形，平均大小为22.5 × 15.2 μm。棘状树突呈放射状延伸，树突野的平均半径额面为214.7 μm，矢状面为171.4 μm。这些形态学特征与丘脑感觉中继核相似。
SummaryIntracellular recordings from thalamic neurons receiving cerebellar inputs were performed under urethane anesthesia in the rat. A total of 64 neurons were recorded intracellularly with micropipettes filled with 4% biocytin solution (dissolved in 0.5 M K-acetate), and cerebellar-induced EPSPs (CN-EPSPs), the membrane resistance and firing properties were analyzed with intracellular current injections. The mean latency of CN-EPSPs was 1.9 ± 0.8 ms and the mean input resistance measured in 10 neurons was 17.6 ± 5.0 MΩ. Thirty-two out of 35 stained neurons were analysed morphologically; 28 of these neurons were located in the VL, and 26 received CN-EPSPs. Their somata were round or polygonal in shape and the mean size was 22.5 × 15.2 μm. They had radially extending spinous dendrites, and the mean radii of the dendritic fields were 214.7 μm in the frontal and 171.4 μm in the sagittal planes. These morphological features were similar to those observed in the sensory relay nucleus of the thalamus.