Seasonal Dynamics of Bathyarchaeota-Dominated Benthic Archaeal Communities Associated with Seagrass (Zostera japonica) Meadows
Seasonal Dynamics of Bathyarchaeota-Dominated Benthic Archaeal Communities Associated with Seagrass (Zostera japonica) Meadows
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DOI：
10.3390/jmse9111304
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发表时间：
2021-11-01
影响因子：
2.9
通讯作者：
Hu, Xiaoke
中科院分区：
文献类型：
--
作者：
Liu, Pengyuan;Zhang, Haikun;Hu, Xiaoke
温带海草生态系统中古菌群落的季节动态及其潜在的生态功能还知之甚少。本研究利用16 SrRNA基因的Miseq序列分析和Tax 4Fun 2功能预测，研究了沿着渤海北方两个海草草甸表层沉积物中底栖古菌的多样性、群落结构和潜在代谢功能的季节变化。总的来说，Crenarchaeota（主要是Bathy-15，Bathy-8和Bathy-6）占主导地位，其次是Thermoplasmatota，Asgardarchaeota和Halobacterota，在α-dilatum和相对丰度方面。在整个古菌群落结构的显着的季节性变化进行了观察。主要门甲烷菌，Nitrosopumilales，和属Methanolobus在春季的比例较高，而MBG-D和Bathyarchaeota分别在夏季和秋季更丰富。Shannon和Simpson的α-D值夏季最高，秋季最低（ANOVA检验，p < 0.05）。盐度、总有机碳和总有机氮是影响整个古菌群落的最重要因素。纤维素和半纤维素的降解潜力在夏季较高，而甲烷代谢潜力在冬季较高。本研究表明，在温带海草生态系统中，季节对调节底栖古菌多样性和功能潜力有很强的影响。
Little is known about the seasonal dynamic of archaeal communities and their potential ecological functions in temperate seagrass ecosystems. In this study, seasonal changes in diversity, community structure, and potential metabolic functions of benthic archaea in surface sediments of two seagrass meadows along the northern Bohai Sea in China were investigated using Miseq sequencing of the 16S rRNA gene and Tax4Fun2 functional prediction. Overall, Crenarchaeota (mainly Bathy-15, Bathy-8, and Bathy-6) dominated, followed by Thermoplasmatota, Asgardarchaeota, and Halobacterota, in terms of alpha diversities and relative abundance. Significant seasonal changes in the entire archaeal community structure were observed. The major phyla Methanobacteria, Nitrosopumilales, and genus Methanolobus had higher proportions in spring, while MBG-D and Bathyarchaeota were more abundant in summer and autumn, respectively. Alpha diversities (Shannon and Simpson) were the highest in summer and the lowest in autumn (ANOVA test, p < 0.05). Salinity, total organic carbon, and total organic nitrogen were the most significant factors influencing the entire archaeal community. Higher cellulose and hemicellulose degradation potentials occurred in summer, while methane metabolism potentials were higher in winter. This study indicated that season had strong effects in modulating benthic archaeal diversity and functional potentials in the temperate seagrass ecosystems.