Effects of cadmium on osteoblasts and osteoclasts in vitro
Effects of cadmium on osteoblasts and osteoclasts in vitro
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镉对体外成骨细胞和破骨细胞的影响
DOI：
10.1016/j.etap.2009.04.010
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发表时间：
2009-09-01
影响因子：
4.3
通讯作者：
Shao, Chunlin
中科院分区：
文献类型：
--
作者：
Chen, Xiao;Zhu, Guoying;Shao, Chunlin
镉(Cd)可能对骨代谢有直接影响，其机制尚不完全清楚。为了探讨镉对骨代谢的影响，从细胞和分子水平观察了镉对体外培养的成骨细胞和破骨细胞的影响。采用序贯酶消化法培养SD大鼠颅骨成骨细胞，分离新生雄性和雌性SD大鼠长骨破骨细胞，将细胞暴露于不同浓度的镉(成骨细胞为0~2.0mU/L，破骨细胞为0.03mU/L)。对成骨细胞进行细胞活力、碱性磷酸酶(ALP)活性和矿化测定。逆转录-聚合酶链式反应(RT-PCR)检测骨保护素(OPG)和核因子-kappaB受体激活剂(RANKL)的表达。破骨细胞经0.03mU·mol/L的Cd染毒72h和120h后，可观察到破骨细胞的数量和凹坑的形成。Cd抑制成骨细胞的活性、碱性磷酸酶活性、矿化和上调RANKL基因的表达。而Cd对Cpg基因表达无明显影响。对于破骨细胞，镉(0.03MU/L)可增加破骨细胞数量(p<；0.05)，促进凹坑形成(p<；0.05)。提示高浓度镉可抑制骨形成，低浓度镉可促进骨吸收。OPG/RANKL可能是镉作用于骨的重要途径。(C)2009爱思唯尔B.V.保留所有权利。
Cadmium (Cd) may have direct effects on bone metabolism and the mechanism is not fully understood. To investigate the effects of Cd on bone metabolism, effects of Cd on osteoblasts and osteoclasts in vitro were observed at cellular and molecular levels. Osteoblasts were cultured by sequential enzyme digestion from Sprague-Dawley rats calvarial bone and osteoclasts were isolated from long bones of new-born male and female Sprague-Dawley rats, and then cells were exposed to different concentrations of Cd (0-2.0 mu mol/L for osteoblasts; 0.03 mu mol/L for osteoclasts). As for osteoblasts, cell viability, alkaline phosphatase (ALP) activity, and mineralization were determined. Osteoprotegerin (OPG) and receptor activator of NF-kappa B ligand (RANKL) were studied via reverse transcription-polymerase chain reaction (RT-PCR). For osteoclasts, after exposure to Cd (0.03 mu mol/L) for 72 h and 120 h, number of osteoclasts and pits formation was observed. Cd inhibited the viability, ALP activity, mineralization and up-regulated RANKL mRNA expression in osteoblasts. But Cd had no obvious effect on CPG mRNA expression. For osteoclasts, cadmium (0.03 mu mol/L) could increase the numbers of osteoclasts (p < 0.05) and enhance pits formation (p < 0.05). These results suggested that Cd could inhibit bone formation at high concentrations and enhance bone resorption at low level. OPG/RANKL may constitute an important pathway of Cd effects on bone. (C) 2009 Elsevier B.V. All rights reserved.