CELL-MOVEMENT AND SHAPE ARE NONRANDOM AND DETERMINED BY INTRACELLULAR, OSCILLATORY ROTATING WAVES IN DICTYOSTELIUM AMEBAS
CELL-MOVEMENT AND SHAPE ARE NONRANDOM AND DETERMINED BY INTRACELLULAR, OSCILLATORY ROTATING WAVES IN DICTYOSTELIUM AMEBAS
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DOI：
10.1016/0303-2647(94)90048-5
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发表时间：
1994-01-01
期刊：
影响因子：
1.6
通讯作者：
VICKER, MG
中科院分区：
文献类型：
--
作者：
KILLICH, T;PLATH, PJ;VICKER, MG
我们提供了真核细胞运动机制的证据。使用计算机支持的视频显微镜研究了盘基网柄菌变形虫的伪足动力学和形状。通过连续圆形图的新方法对细胞外围进行检查，揭示了明确的经典波模式，这表明存在内在的细胞内振荡。这些图案是由旋转振荡波 (ROWS) 的自组织、叠加、谐波模式的传输生成的。这些波可能与细胞内肌动蛋白聚合和解聚的动力学有关。在 10 分钟的运动过程中，使用围绕细胞边界每 10 度的点对一个细胞进行 Karhunen-Loeve 扩展。结果表明，只需要 2-3 种模式即可描述细胞运动和形状的最基本特征。基于此分析，开发了一个波模型，它准确地模拟了这段时间内细胞运动和形状的动态。该模型通过 Karhunen-Loeve 变换重建细胞的动态形式进行了测试。在该重建和实际细胞轮廓之间没有检测到差异。尽管细胞运动和形状迄今为止被认为是随机的，但我们的结果表明，ROWS 决定伪足的时空表达，从而非随机地控制细胞形状和运动。
We present evidence for a mechanism of eukaryotic cell movement. The pseudopodial dynamics and shape of Dictyostelium discoideum amoebae were investigated using computer-supported video microscopy, An examination of the cell periphery by the novel method of serial circular maps revealed explicit, classical wave patterns, which indicate the existence of intrinsic intracellular oscillations. The patterns are generated by the transit of self-organized, superpositioned, harmonic modes of rotating oscillatory waves (ROWS). These waves are probably associated with the dynamics of intracellular actin polymerisation and depolymerisation. A Karhunen-Loeve expansion was conducted on one cell during 10 min of locomotion using points each 10 degrees around the cell's boundary. The results show that only 2-3 modes are necessary to describe the most essential features of cell movement and shape. Based on this analysis, a wave model was developed, which accurately simulates the dynamics of cell movement and shape during this time. The model was tested by reconstructing the cell's dynamical form by means of the Karhunen-Loeve transform. No difference was detected between this reconstruction and the actual cell outline. Although cell movement and shape have hitherto been viewed as random, our results demonstrate that ROWS determine the spatio-temporal expression of pseudopodia, and consequently govern cell shape and movement, non-randomly.