C-erbB-2 gene product, a membrane protein commonly expressed on human fetal epithelial cells.
C-erbB-2 gene product, a membrane protein commonly expressed on human fetal epithelial cells.
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C-erbB-2 基因产物，一种通常在人类胎儿上皮细胞上表达的膜蛋白。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Tadashi Yamamoto
中科院分区：
文献类型：
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作者：
S. Mori;T. Akiyama;Y. Yamada;Y. Morishita;I. Sugawara;K. Toyoshima;Tadashi Yamamoto
c-erbB -2是一种人类原癌基因，与已知的原癌基因c-erbB具有同源性。c-erbB-2基因在一些人类腺癌中被扩增和过表达。它在正常人类胎儿肾脏、肺和肝脏中表达的RNA水平也有报道。本研究采用ABC免疫组织学方法和Western blot免疫化学方法研究了3例妊娠9、14和24周的人流产胎儿组织中c-erbB-2基因产物在蛋白水平上的分布。采用与预测胞浆内结构域部分对应的合成多肽免疫兔获得的多克隆抗体。妊娠期大部分上皮细胞的膜均有较强的免疫反应性，包括肾盂和输尿管的移行细胞，胃肠道、肾小管、支气管和胰腺的腺细胞，口腔、气管和食道的分层上皮。在这些细胞的基底外侧观察到的反应比在顶端观察到的反应强烈得多。肝、肾上腺、横纹肌和平滑肌、脑、内皮细胞和成纤维细胞均未见免疫反应性。Western blot分析证实，c-erbB-2基因产物在胎儿肾脏和肠道中表达增加，但在大脑中没有表达增加。由于该蛋白在除肾小管外的正常成人上皮细胞中表达较差，因此目前的结果表明该蛋白是一种膜相关受体蛋白，控制着胎儿上皮的某些特异性反应。
C-erbB-2 is a human proto-oncogene which has homologies with the well known proto-oncogene c-erbB. The c-erbB-2 gene is amplified and overexpressed in some human adenocarcinomas. Its expression, in terms of RNA levels in normal human fetal kidney, lung and liver, has also been reported. In the present study, various fetal tissues from three human abortuses obtained at 9, 14 and 24 weeks of gestation, were studied immunohistologically by the ABC method and immunochemically by Western blot analysis for the distribution of c-erbB-2 gene product at the protein level. A polyclonal antibody raised in rabbit by immunization with a synthetic polypeptide corresponding to part of the predicted intracytoplasmic domain was used. Strong immunoreactivity was observed on the membrane of most of the epithelial cells examined, including transitional cells of the renal pelvis and ureter, glandular cells of the gastrointestinal tract, renal tubuli, bronchi and pancreas, and stratified epithelium of the oral cavity, trachea and esophagus in this gestational period. A much more intense reaction was observed on the basolateral sides than on the apical side of these cells. No immunoreactivity was found in the liver, adrenal gland, striated and smooth muscles, brain, endothelium or fibroblasts. Western blot analysis confirmed increased expression of the c-erbB-2 gene product in fetal kidney and intestine but not in the brain. As the protein seems to be poorly expressed in normal adult epithelial cells except for renal tubuli, the present results suggest that the protein is a membrane-associated receptor protein which controls some specific reaction of fetal epithelium.