Prostaglandin dehydrogenase (PGDH) in granulosa cells of primate periovulatory follicles is regulated by the ovulatory gonadotropin surge via multiple G proteins.
Prostaglandin dehydrogenase (PGDH) in granulosa cells of primate periovulatory follicles is regulated by the ovulatory gonadotropin surge via multiple G proteins.
复制标题
灵长类动物排卵期卵泡颗粒细胞中的前列腺素脱氢酶 (PGDH) 通过多种 G 蛋白受到排卵促性腺激素激增的调节。
DOI：
10.1016/j.mce.2010.12.016
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发表时间：
2011
影响因子：
4.1
通讯作者：
Duffy,DianeM
中科院分区：
文献类型：
--
作者：
Duffy,DianeM
排卵期促性腺激素激增增加了颗粒细胞前列腺素的合成和前列腺素脱氢酶，前列腺素脱氢酶是负责前列腺素代谢的关键酶。为了研究促性腺激素对灵长类卵泡中促性腺激素的调节作用，在40小时的排卵间隔内获得了猕猴颗粒细胞。人绒毛膜促性腺激素(HCG)刺激前前列腺素脱氢酶活性较低，hCG刺激后12-24小时达到高峰，36小时后再次下降。人绒毛膜促性腺激素(HCG)体外培养的颗粒细胞表现出与PGDH相似的时间模式。黄体生成素/卵泡刺激素受体可以利用多种信号通路来调节细胞内事件。促性腺激素刺激的cAMP似乎主要通过Epas来增加PGDHmRNA、蛋白和活性。相反，在排卵后期，PLC对PKC的激活可能会降低PGDHmRNA、蛋白和活性。前列腺素脱氢酶活性先升高后降低，可能会推迟前列腺素在卵泡中的积累，直到排卵周期的晚期，有助于灵长类动物适时排卵。
The ovulatory gonadotropin surge increases granulosa cell prostaglandin synthesis as well as prostaglandin dehydrogenase (PGDH), the key enzyme responsible for prostaglandin metabolism. To investigate gonadotropin regulation of PGDH in the primate follicle, monkey granulosa cells were obtained across the 40-h periovulatory interval. PGDH activity was low before the ovulatory hCG stimulus, peaked 12–24h after hCG, and was low again 36h after hCG administration. Granulosa cells maintained in vitro with hCG showed a similar temporal pattern of PGDH. The LH/CG receptor can utilize multiple signaling pathways to regulate intracellular events. Gonadotropin-stimulated cAMP appears to act primarily via the Epacs to increase PGDH mRNA, protein, and activity. In contrast, PLC activation of PKC likely decreases PGDH mRNA, protein, and activity late in the periovulatory interval. Increased, then decreased PGDH activity may delay accumulation of prostaglandins in the follicle until late in the periovulatory interval, contributing to timely ovulation in primates.