Bovine serum albumin enhances calcium currents in chicken granulosa cells.
Bovine serum albumin enhances calcium currents in chicken granulosa cells.
复制标题
牛血清白蛋白增强鸡颗粒细胞中的钙电流。
DOI：
10.1016/0303-7207(93)90048-o
复制
发表时间：
1993
影响因子：
4.1
通讯作者：
Asem,EK
中科院分区：
文献类型：
--
作者：
Chiang,M;Strong,JA;Asem,EK
采用制霉菌素穿孔法和常规全细胞膜片钳技术，观察了牛血清白蛋白(BSA)对鸡颗粒细胞钙电流的影响。在电压钳条件下，两种方法的去极化电压阶跃都能诱发内向钙电流。用穿孔贴片技术测量的钙电流的时间和电压依赖关系与常规全细胞记录的结果相似。市售牛血清白蛋白和基本不含脂肪酸的牛血清白蛋白均能迅速增强钙电流的幅度。然而，不含脂肪酸的BSA的效力更强，与饱和浓度的油酸孵育后，其效力大大降低。这些数据表明，BSA是孵育液中的常见成分，可以影响颗粒细胞质膜上的离子通道。
The effect of bovine serum albumin (BSA) on Ca2+currents in chicken granulosa cells was examined using both the nystatin-perforated and the conventional whole cell patch clamp techniques. Under voltage-clamp conditions, depolarizing voltage steps evoked inward Ca2+currents with both methods. The time- and voltage-dependence of Ca2+currents measured with the perforated patch technique was similar to those obtained with conventional whole cell recording. Commercially prepared BSA and essentially fatty acid free BSA both rapidly enhanced the amplitude of Ca2+currents. However, the fatty acid free BSA was more potent, and its potency was greatly reduced by incubation with saturating concentrations of oleic acid. These data show that BSA, a common constituent of incubation media, can influence ion channels in the plasma membrane of granulosa cells.