Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications
Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications
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DOI：
10.1021/acsnano.6b07808
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发表时间：
2017-05-01
期刊：
影响因子：
17.1
通讯作者：
Xiao, Yin
中科院分区：
文献类型：
--
作者：
Chen, Zetao;Bachhuka, Akash;Xiao, Yin
骨免疫调节表明调节有利的骨免疫环境对于材料介导的骨再生成功的重要性。纳米形貌因其对增强成骨分化的积极作用而被认为是开发先进骨材料的重要策略。除了对成骨细胞谱系细胞的直接影响外，纳米拓扑结构还在调节免疫反应中发挥着至关重要的作用，这使得利用其免疫调节特性创造有利的骨免疫环境成为可能。因此，本研究的目的是推进纳米形貌在骨免疫调节特性方面的应用，旨在进一步阐明这一领域。我们发现，调整纳米形貌的表面化学（胺或丙烯酸）和规模（16、38和68 nm）可显着调节骨免疫环境，包括炎症细胞因子、破骨细胞活性以及成骨、血管生成和纤维生成因子表达的变化。生成的骨免疫环境显着影响骨髓基质细胞的成骨分化，其中羧酸定制的 68 nm 表面纳米形貌提供了最有希望的结果。这项研究表明，可以通过调整化学和纳米形貌来操纵骨免疫调节，这意味着应用“纳米工程表面”来开发具有良好骨免疫调节特性的先进骨生物材料是一种有价值的策略。
Osteoimmunomodulation has informed the importance of modulating a favorable osteoimmune environment for successful materials-mediated bone regeneration. Nanotopography is regarded as a valuable strategy for developing advanced bone materials, due to its positive effects on enhancing osteogenic differentiation. In addition to this direct effect on osteoblastic lineage cells, nanotopography also plays a vital role in regulating immune responses, which makes it possible to utilize its immunomodulatory properties to create a favorable osteoimmune environment. Therefore, the aim of this study was to advance the applications of nanotopography with respect to its osteoimmunomodulatory properties, aiming to shed further light on this field. We found that tuning the surface chemistry (amine or acrylic acid) and scale of the nanotopography (16, 38, and 68 nm) significantly modulated the osteoimmune environment, including changes in the expression of inflammatory cytokines, osteoclastic activities, and osteogenic, angiogenic, and fibrogenic factors. The generated osteoimmune environment significantly affected the osteogenic differentiation of bone marrow stromal cells, with carboxyl acid-tailored 68 nm surface nanotopography offering the most promising outcome. This study demonstrated that the osteoimmunomodulation could be manipulated via tuning the chemistry and nanotopography, which implied a valuable strategy to apply a "nanoengineered surface" for the development of advanced bone biomaterials with favorable osteoimmunomodulatory properties.