INTERACTION BETWEEN BARLEY AND MIXED CULTURES OF NITROGEN-FIXING AND PHOSPHATE-SOLUBILIZING BACTERIA
INTERACTION BETWEEN BARLEY AND MIXED CULTURES OF NITROGEN-FIXING AND PHOSPHATE-SOLUBILIZING BACTERIA
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DOI：
10.1007/bf00155515
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发表时间：
1995-06-01
期刊：
影响因子：
4.9
通讯作者：
CHUVARLIYEVA, CV
中科院分区：
文献类型：
--
作者：
BELIMOV, AA;KOJEMIAKOV, AP;CHUVARLIYEVA, CV
通过盆栽试验，研究了固氮菌Azospirillum lipoferum 137、mysorens节杆菌7和磷溶菌Agrobacterium radiobacter 10分别接种纯培养和混合培养对2个大麦品种生长和矿质营养的影响。单用A. lipoferum 137 + A. radiobacter 10和A. lipoferum 137 + A. mysorens 7混合接种对两种大麦品种的籽粒产量均有显著的正影响。当A. lipoferum 137与A. radiobacter 10联合培养时，根上和批培养中乙炔还原活性显著提高。利用N-15同位素稀释技术证实，这些混合培养显著增加了植株氮肥的积累。菌株a . radiobacter 10促进了植物对磷肥的较好积累，菌株a . mysorens 7提高了植物组织中总磷含量。联合接种对植物发育的积极作用在土壤氮素不足时达到最大。综上所述，细菌混合接种能使植株获得更均衡的营养，提高根系对氮磷的吸收是植物与细菌相互作用的主要机制。引入的细菌能够在大麦的根面上活跃地定植。根际细菌混合物各组分之间不存在种间竞争或拮抗作用。菌株A. mysorens 7和A. radiobacter 10提高了A. lipoferum 137在酸性土壤中的生存能力。对3个大麦品种进行的田间试验证实，与单一培养相比，混合接种显著提高了籽粒产量和植株氮素营养。
Pot experiments were carried out to investigate the effect of inoculation with pure and mixed cultures of nitrogen fixers Azospirillum lipoferum 137, Arthrobacter mysorens 7 and the phosphate-solubilizing strain Agrobacterium radiobacter 10 on growth and mineral nutrition of two barley cultivars. A significant positive effect on grain yield both of the studied barley cultivars was obtained after inoculation with mixtures of A. lipoferum 137 + A. radiobacter 10 and A. lipoferum 137 + A. mysorens 7 only. The acetylene reduction activity on roots or in batch culture was significantly higher when A. lipoferum 137 and A. radiobacter 10 were combined. Using N-15 isotope dilution technique it was established that these mixed cultures significantly increased the accumulation of nitrogen fertilizer in the plants. The strain A. radiobacter 10 promoted a better accumulation of phosphorus fertilizer by plants and A. mysorens 7 increased the total phosphorus content in plant tissues. The maximum positive effect of joint inoculation on plant development was observed when the combined nitrogen in soil was in short supply. It was concluded that inoculation with bacterial mixtures provided a more balanced nutrition for the plants and the improvement in root uptake of nitrogen and phosphorus was the major mechanism of interaction between plants and bacteria. The introduced bacteria were able to colonize actively the rhizoplane of barley. No interspecific competition or antagonism were established between components of the bacterial mixtures in the rhizoplane. The strains A. mysorens 7 and A. radiobacter 10 improved viability of A. lipoferum 137 when the plants were grown in acid soil. Field experiments carried out on 3 barley cultivars confirmed the assertion that inoculation with mixed cultures significantly increases the grain yield and nitrogenous nutrition of plants as compared with single cultures.