Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae
Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae
复制标题
DOI：
10.1101/gad.1039602
复制
发表时间：
2002-12-01
影响因子：
10.5
通讯作者：
Snyder, M
中科院分区：
文献类型：
--
作者：
Horak, CE;Luscombe, NM;Snyder, M
在酿酒酵母的酵母菌中，SBF（（s）在bar Wi4-Swi6细胞周期盒（b）下，在bar inding系数下）和MBF（（M）在bar lui（b）下，在bar Inding系数下）是调节的主要转录因子细胞周期的开始，即DNA复制，芽生长启动和主轴杆体（SPB）重复的时间。这两个因素与235个基因的启动子结合，但在细胞周期的G1相的峰值转录水平上游的启动子的四分之一结合。已知对于G1/S事件至关重要的几个功能类别，例如SPB重复/迁移和DNA合成，在SBF和MBF基因靶标列表中代表不足。 SBF结合了其他几种转录因子的启动子，包括HCM1，PLM2，POG1，TOS4，TOS8，TOS8，TYE7，YAP5，YHP1和YOX1。在这里，我们证明了这些因素是使用独立测定的SBF的靶标。为了进一步阐明调节G1到S相进展的转录电路，这些因素被表达标记，并通过CHIP-CHIP分析确定其结合靶标。这些因素将基因的启动子与G1/S事件中的作用结合，包括DNA复制，芽生长和纺锤形复合物的形成，以及线粒体功能，转录和蛋白质合成的一般活性。尽管这些因素与MBF和SBF之间存在功能重叠，但这些因素中的每一个都具有不同的功能作用。这些因素中的大多数结合了被称为细胞周期的其他转录因子的启动子，对细胞周期进程和分化过程很重要，表明复杂的转录因子网络会协调启动新细胞周期的各种活动。
In the yeast Saccharomyces cerevisiae, SBF ((S) under bar wi4-Swi6 cell cycle box (b) under bar inding factor) and MBF ((M) under bar luI (b) under bar inding factor) are the major transcription factors regulating the START of the cell cycle, a time just before DNA replication, bud growth initiation, and spindle pole body (SPB) duplication. These two factors bind to the promoters of 235 genes, but bind less than a quarter of the promoters upstream of genes with peak transcript levels at the G1 phase of the cell cycle. Several functional categories, which are known to be crucial for G1/S events, such as SPB duplication/migration and DNA synthesis, are under-represented in the list of SBF and MBF gene targets. SBF binds the promoters of several other transcription factors, including HCM1, PLM2, POG1, TOS4, TOS8, TYE7, YAP5, YHP1, and YOX1. Here, we demonstrate that these factors are targets of SBF using an independent assay. To further elucidate the transcriptional circuitry that regulates the G1-to-S-phase progression, these factors were epitope-tagged and their binding targets were identified by chIp-chip analysis. These factors bind the promoters of genes with roles in G1/S events including DNA replication, bud growth, and spindle pole complex formation, as well as the general activities of mitochondrial function, transcription, and protein synthesis. Although functional overlap exists between these factors and MBF and SBF, each of these factors has distinct functional roles. Most of these factors bind the promoters of other transcription factors known to be cell cycle regulated or known to be important for cell cycle progression and differentiation processes indicating that a complex network of transcription factors coordinates the diverse activities that initiate a new cell cycle.